MmuINT0103389 @ mm10
Intron Retention
Gene
ENSMUSG00000092009 | Myh15
Description
myosin, heavy chain 15 [Source:MGI Symbol;Acc:MGI:3643515]
Coordinates
chr16:49141382-49143006:+
Coord C1 exon
chr16:49141382-49141527
Coord A exon
chr16:49141528-49142915
Coord C2 exon
chr16:49142916-49143006
Length
1388 bp
Sequences
Splice sites
5' ss Seq
GAAGTAAGC
5' ss Score
6.95
3' ss Seq
TGTCATATTTCTCAAAACAGGAA
3' ss Score
8.65
Exon sequences
Seq C1 exon
TTGGAAGGTGACCTTGAACGGGAGAGGAAAGCCAGAATGAAGTGTGAGAGGGAGAAGCGCAAGCTGCAAGATGAGTTAAAGATGAACCAGGAGGGTGCTGAGAATCTGGAGAGCAGCCGGCAGAAGCTGGCAGAGCAGCTGAGGAA
Seq A exon
GTAAGCATCTTTCCACTAACTGCCAAGGGATTCTGGGAAAATCTCAGCGCTGGGAGGAGCAAGGCTCTCATTTCATTTAGCAATAGGTTTCTAGGCCAGAGCCAGATAGCTCAACAGCCAAGACAGTGACTAGAAGGTTGGAAAATGGAGAGGTTTTACTTGGTGGACTCTATTACATAGTAGCAAAAATAAAGACATAATCCACAGGATATAACAAAAAGAAACCACCTAGAAGATGAGAATTTTGGAAGGTATAAGGCATGTGAGAGGTGAATTATGGGTATAATTGAGTGTACAAAAATTGGTTGATTGCTTTTACTTGTGATAAGACCAATAGCAACATGAACATGCCCAAATCCTGAAGCTTGAAAAGCTGTATGGTCACTTAAATAATATCACCTTTTTGTTAGATACACCTCCATTAATACTTATGTTGGGTACTCATTTCCAAAATATGTAAACATTGATTCCTAAAACCTAAAATTATAATTACACAAAGTGATAAAAGTTATGGAAAATTTCAATAAGTTCCCTTGCATTTCTTGTACCATATGGAAGTGATGGGAAGATGACTTCCTGTTTGGATAATACTTAAAGGTTTAGATTCTACCAAAAATAGGAAAAGTGATTTTAAGATACAAGGTTAAGACTGTAAAACATATAAAAATTGGGGTCAACTGAGCAGCTTGAGTTTTTAGCTGCGTTTGCATGCATGCATTGGCCTCAAGCCTGTTCTTGGTGATGCCTGACTAATAGTCTCCTCTTAATTTAGTATACATGACATGTGTCTGGGCTTACCTTAAGAGTGTAATGGAACAGTATGATAGAAGATGATGACAGTGTTTTCTTTGCCATCAGTTTTGAAAATTATGTCAGTTTGGCAAAGTGACGATTATACTCCATGCCTCTGTAGGCAGTATGTCAGTGTTTGAAAATGTTGAATATGTTTCATAGGCTTATTATAAAGCAATTTCCATGTGGGACCATGTAGAAGCATGCCCCTTAGATTGTTTGGGTCTTGTTATGCTATATATCTGTACATAAAATTCATCCAATATTATAGATTCTACAGTAAAGAAAATGGAAGTATCTCTAGTATTACACAGACATACACATAGAAACACACACACACACACACACACACACACACACACACACACACCATTTGTTGAGTACCTAGGGAAAAGTTCAGAAGGACACACACCAAACATGTGATCATGGTTTGTAGAAAGAGATCAGGAATTATGTAGTACTCATGATGACTTCAGATGTACATGGAATGATTATATTTTTTAAAGTAGGAATTCATATGTTATTCCATAACTTCAAAGTATATAAATATGTTTTATTATTCTGATAGATTATCTTTGTCATATTTCTCAAAACAG
Seq C2 exon
GAAGGAGTTTGAAATGGGTCAGATGAATTCAAAAGTGGAGAATGAGAAGAACCAGGTGTCTCAGCTTCAGAAGATGGTGAAAGAGCTTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000092009:ENSMUST00000168680:25
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.837 A=NA C2=0.839
Domain overlap (PFAM):
C1:
PF087025=Fib_alpha=FE(42.9=100),PF0157614=Myosin_tail_1=PU(1.6=28.6)
A:
NA
C2:
PF087025=Fib_alpha=FE(26.8=100),PF0157614=Myosin_tail_1=FE(3.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGGTGACCTTGAACGGGAG
R:
AGCTCTTTCACCATCTTCTGAAGC
Band lengths:
230-1618
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types