Special

MmuINT0103437 @ mm10

Intron Retention

Gene
ENSMUSG00000033196 | Myh2
Description
myosin, heavy polypeptide 2, skeletal muscle, adult [Source:MGI Symbol;Acc:MGI:1339710]
Coordinates
chr11:67192667-67193062:+
Coord C1 exon
chr11:67192667-67192832
Coord A exon
chr11:67192833-67192937
Coord C2 exon
chr11:67192938-67193062
Length
105 bp
Sequences
Splice sites
5' ss Seq
AGCGTAAGG
5' ss Score
5.87
3' ss Seq
GCTTTTCACCCTATTTACAGAGG
3' ss Score
7.28
Exon sequences
Seq C1 exon
ATCCTGGCAGAGTGGAAGCAGAAGTATGAGGAAACCCACGCTGAGCTCGAGGCATCCCAGAAGGAGGCCCGCTCCCTGGGCACTGAGCTCTTCAAGATGAAGAATGCCTACGAGGAGTCTCTGGATCAGCTAGAAACCCTGAAGCGAGAGAATAAGAACTTACAGC
Seq A exon
GTAAGGCCCTTTCTCTCTCAACAGTACCCATGACTTGGAGGGAACACTCGCTCTTCTATGCCATGTCTTGACCACACCCTTACCTGCTTTTCACCCTATTTACAG
Seq C2 exon
AGGAGATTTCTGACCTCACGGAACAGATTGCAGAAGGAGGAAAGCGCATCCACGAACTGGAGAAAATTAAGAAACAAGTCGAACAAGAGAAGTGTGAACTTCAGGCTGCTCTAGAAGAAGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033196:ENSMUST00000018641:31
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.339 A=NA C2=0.238
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF0003816=Filament=FE(19.3=100),PF045827=Reo_sigmaC=FE(36.9=100)

							
A:
NA

							
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF0003816=Filament=FE(14.4=100),PF045827=Reo_sigmaC=PD(22.1=78.6)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000018641





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000129544
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr17:10525349-10525450 
LOW PSI
HsaINT0107706
(MYH2)
Human
(hg19)
chr17:10428666-10428767 
LOW PSI
HsaINT0107706
(MYH2)
Rat
(rn6)
chr10:53733348-53733447 
ALTERNATIVE
RnoINT0096288
(Myh1)
Cow
(bosTau6)
chr19:30142716-30142814 
ALTERNATIVE
BtaINT0097277
(MYH2)
Chicken
(galGal4)
chr18:476919-477090 
Chicken
(galGal3)
chr18:485691-485862 
GgaINT0128387
(Q90900_CHICK)
Zebrafish
(danRer10)
chr24:41065066-41065168 
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GTATGAGGAAACCCACGCTGA

				
R: 
GAGCAGCCTGAAGTTCACACT

				
Band lengths: 
254-359

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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