MmuINT0103484 @ mm10
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67082282-67083007:+
Coord C1 exon
chr11:67082282-67082425
Coord A exon
chr11:67082426-67082850
Coord C2 exon
chr11:67082851-67083007
Length
425 bp
Sequences
Splice sites
5' ss Seq
TACGTGAGT
5' ss Score
7.77
3' ss Seq
TATTTTTTCCCCTCCCATAGACC
3' ss Score
10.66
Exon sequences
Seq C1 exon
ACCCTGGTGGTTAAGCCAGAGGACGTGTATGCCATGAACCCACCCAAGTTTGACAAGATTGAGGACATGGCCATGCTGACCCACCTGAACGAGCCCGCTGTGCTGTACAACCTCAAGGACCGATACACGTCCTGGATGATCTAC
Seq A exon
GTGAGTGGCTACTACCCAACAGGCTATGCTGGGACATCAGCTGGGGGGCAGAGGAGTGGGGGAGGGTGTCTTCAAAGATTCCTGTTATCTGGCTGAAAGAGAGGAGGTGAAGTAGAGAAGTGAATGACCAGAAAAGCCAGAGATGAATGGGATGGGGAGGGGAGGGGCTGGAGGAAGGGAGGTAGAAAGGGGGGAAGTGGAGGAGGAGCTAAGGTTGGGAGGAGGAGCCCCAAAGAGTAAGAAAGCTGAGCATGGTATTAGTTGTGGGAGGACTGGTTGCTTCCTTCCTTGAATTTCAGTTACTTCATTCATCAATAGAAATCATGATTTCTTTATGAGTAATCAAAAGAAGCCTGGGATATTAGTAAAGGCAACACACACACATATCATATCATATATATATATTATTTTTTCCCCTCCCATAG
Seq C2 exon
ACCTATTCAGGCCTCTTCTGTGTCACTGTCAACCCCTACAAATGGCTGCCAGTGTACAACCCCGAGGTGGTGGACGGCTACCGAGGCAAAAAACGCCAGGAGGCTCCGCCCCACATCTTCTCCATCTCCGACAACGCCTACCAGTTCATGCTGACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908:ENSMUST00000108689:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.021 A=NA C2=0.057
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
A:
NA
C2:
PF0006316=Myosin_head=FE(7.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGTTAAGCCAGAGGACGTGT
R:
CAGTCAGCATGAACTGGTAGGC
Band lengths:
294-719
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types