MmuINT0103503 @ mm9
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67064003-67064509:+
Coord C1 exon
chr11:67064003-67064139
Coord A exon
chr11:67064140-67064253
Coord C2 exon
chr11:67064254-67064509
Length
114 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGG
5' ss Score
8.41
3' ss Seq
CTGTTTTTCTCCTTCCACAGAGA
3' ss Score
11.62
Exon sequences
Seq C1 exon
GTTTTCTTTAAAGCCGGCCTGTTGGGAACTCTAGAGGAGATGCGAGATGAAAAGCTGGCTCAGCTCATCACCCGCACTCAAGCCGTGTGCAGAGGGTACCTGATGAGGGTGGAGTTCAAAAAGATGATGGAGAGGAG
Seq A exon
GTGAGGAGCAAAGAGTGTCTGCCTTTCCTACCTCTTTCATCTCGGAATCCAGAGCACGTGTGATGGTCACTTCACAATCTTTGCCCACTTGTTTCTGTTTTTCTCCTTCCACAG
Seq C2 exon
AGAGTCCATCTTCTGCATTCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTATTTCAAGATCAAACCTCTGCTGAAGAGCGCGGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGATTTTGAGAAGGCCAAGGAGGATCTGGCTAAGTCAGAGGCAAAAAGGAAGGAACTAGAAGAAAAGATGGTAGCTTTGATGCAGGAGAAAAATGACCTGCAGCTCCAGGTTCAAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-Myh4:NM_010855:20
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.130
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=6.5),PF0061222=IQ=WD(100=43.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=PU(28.5=64.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGCCTGTTGGGAACTCTAGA
R:
TTGGCCATCTCCTTCTCGGTC
Band lengths:
252-366
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: