MmuINT0103510 @ mm10
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67254000-67254576:+
Coord C1 exon
chr11:67254000-67254126
Coord A exon
chr11:67254127-67254457
Coord C2 exon
chr11:67254458-67254576
Length
331 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
3' ss Seq
ATTTGTGTTTCCTCTCTCAGGCG
3' ss Score
10.38
Exon sequences
Seq C1 exon
GGGAACCTTGAGAAGATGTGCCGCACCCTGGAGGACCAACTGAGTGAAGTGAAGACCAAGGAGGAGGAGCAGCAGCGCCTGATCAATGAACTGTCAACCCAGAAGGCACGCTTGCACACAGAGTCAG
Seq A exon
GTCAGTCTGTGCCACACTAGCCTGGGAACAGCAAAGCATGAGAGGTCTGCCCGATCCTGGACGCTGGGGTCTTGCCCTGCACAATGTGTACAAGAGATGGTTACAAATGAATGACTCTAAGCTATGATTAGAGGCGAGTTGGGAAATGAACCTCTTGGGCTTGGGAAAGTTTTGTTACCTCCATAACCCTCAAGAATCCCCCATTCTACAATACCAAAGAAAGCAGTTCACGAACATTCTGGAGACACTCTTTGCACTCTATGAATAAGGAGGGTCGTATTCTGGTCCACCTGAGAGAAGAGAGTTCAATCATTTGTGTTTCCTCTCTCAG
Seq C2 exon
GCGAGTTTTCCCGACAGCTGGATGAAAAAGACGCTATGGTTTCTCAGCTTTCCCGAGGCAAACAAGCATTTACACAACAGATTGAGGAACTAAAGAGGCAGCTAGAGGAAGAGTCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003:ENSMUST00000018632:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.825
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.9=100),PF059116=DUF869=PU(30.3=62.8),PF0003816=Filament=PU(4.2=30.2)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.5=100),PF059116=DUF869=FE(43.8=100),PF0003816=Filament=FE(12.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCTTGAGAAGATGTGCCGC
R:
TGGACTCTTCCTCTAGCTGCC
Band lengths:
241-572
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types