MmuINT0103512 @ mm9
Intron Retention
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67068179-67068984:+
Coord C1 exon
chr11:67068179-67068375
Coord A exon
chr11:67068376-67068800
Coord C2 exon
chr11:67068801-67068984
Length
425 bp
Sequences
Splice sites
5' ss Seq
CAAGTATGG
5' ss Score
4.99
3' ss Seq
CGCACTGACCGCCACCCCAGGAA
3' ss Score
4.4
Exon sequences
Seq C1 exon
GCCAAGAACGCACTGGCCCACGCCCTGCAGTCAGCTCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAAGAGGAGCAGGAAGCCAAGGCTGAGCTGCAGAGGGCCATGTCCAAGGCCAACAGCGAGGTAGCCCAGTGGAGGACGAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAAGAGGCCAA
Seq A exon
GTATGGGTTTCAATGAATTAGACAGAAAGAAACTGAAGTAAAGGAAAAGGGAAGGAAAATGGGAAGGAATAATTGTACCAGGAAGAGCATCAAATAATTTCACAGCTTCCATATCTATACAGTTAAGAGGACCATGTACTCAGTCTAGGATCCCACTAGACCCTAGTGAAGAAATGCATCGTTTCCCCTCCCCCTTCTCTCTGGTTTCATAGATATGTTTGAAATAGACACTTCAGATGGAAAAGAGCTCCCCTGGGAAATCAGAATTCTGGGAGAGATAAAATGGTCACTAAGAAGTAATTCTGGAAAAACCTATGGGCACAGAGAAATAAGAGATCTTAGGGGAAAACAGACTTCCCTAATCAGCGGATAGGTAGAGTTCCTTCTGTTAACTTTGAGTCTTCTCGCACTGACCGCCACCCCAG
Seq C2 exon
GAAGAAGCTGGCTCAGCGTCTGCAGGATGCTGAGGAACATGTAGAAGCTGTGAATTCCAAATGCGCTTCTCTTGAAAAGACGAAGCAGCGGCTTCAGAATGAAGTGGAGGACCTCATGATTGACGTGGAGAGGTCTAACGCTGCCTGCGCTGCGCTTGATAAGAAGCAGAGGAACTTTGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-Myh4:NM_010855:29
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.636 A=NA C2=0.290
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(7.6=100),PF059116=DUF869=PD(23.6=31.8),PF0003816=Filament=FE(21.0=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.1=100),PF0003816=Filament=FE(19.7=100),PF045827=Reo_sigmaC=PU(35.4=82.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGAACGCACTGGCCCAC
R:
CAGGCAGCGTTAGACCTCTCC
Band lengths:
342-767
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: