Special

MmuINT0103587 @ mm9

Intron Retention

Gene
ENSMUSG00000053093 | Myh7
Description
myosin, heavy polypeptide 7, cardiac muscle, beta [Source:MGI Symbol;Acc:MGI:2155600]
Coordinates
chr14:55597420-55597830:-
Coord C1 exon
chr14:55597704-55597830
Coord A exon
chr14:55597539-55597703
Coord C2 exon
chr14:55597420-55597538
Length
165 bp
Sequences
Splice sites
5' ss Seq
ATGGTGAGC
5' ss Score
8.27
3' ss Seq
GTACCTTGGTGCCTCCCCAGGGG
3' ss Score
7.09
Exon sequences
Seq C1 exon
GCTAACCTGGAGAAGATGTGCCGGACCTTGGAAGACCAGATGAATGAGCACCGGAGCAAGGCCGAGGAGACGCAGCGTTCTGTCAATGACCTCACCAGCCAGCGGGCCAAGCTGCAGACAGAGAATG
Seq A exon
GTGAGCCCAGGGCTGGAGCTCCATGGGTCCCAACCTCCTGAGCCATCCTAGCCTTGGGTGTACATGCTCCTCATATGCCCACAGCCTCTCCAGAGAAGGGAGCCTAAGAGTTAGGGGCAGGGTTGAGGGTAGAGAGGGACAGAAGGTACCTTGGTGCCTCCCCAG
Seq C2 exon
GGGAGCTGTCCCGGCAGCTGGACGAGAAGGAGGCTCTGATCTCTCAGCTAACCCGAGGCAAGCTCACATATACACAGCAGCTGGAGGACCTCAAGAGGCAACTGGAGGAGGAGGTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053093-Myh7:NM_080728:29
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.860 A=NA C2=0.325
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0003816=Filament=FE(14.8=100),PF0157614=Myosin_tail_1=FE(4.9=100),PF0003816=Filament=PU(2.6=18.6)

							
A:
NA

							
C2:
PF0003816=Filament=PD(4.2=30.0),PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=FE(12.7=100)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000099867





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000126840
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr14:23419296-23419482 
ALTERNATIVE
HsaINT0107856
(MYH7)
Human
(hg19)
chr14:23888505-23888691 
LOW PSI
HsaINT0107856
(MYH7)
Rat
(rn6)
chr15:33641975-33642138 
LOW PSI
RnoINT0096536
(Myh7)
Cow
(bosTau6)
chr19:30144400-30145075 
LOW PSI
BtaINT0097273
(MYH2)
Chicken
(galGal4)
chr18:623664-623768 
Chicken
(galGal3)
chr18:442166-442371 
LOW PSI
GgaINT0127796
([1])
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCTAACCTGGAGAAGATGTGCC

				
R: 
TTGACCTCCTCCTCCAGTTGC

				
Band lengths: 
245-410

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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