MmuINT0103665 @ mm10
Intron Retention
Gene
ENSMUSG00000055775 | Myh8
Description
myosin, heavy polypeptide 8, skeletal muscle, perinatal [Source:MGI Symbol;Acc:MGI:1339712]
Coordinates
chr11:67299271-67299831:+
Coord C1 exon
chr11:67299271-67299397
Coord A exon
chr11:67299398-67299712
Coord C2 exon
chr11:67299713-67299831
Length
315 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGAG
5' ss Score
7.1
3' ss Seq
CCCACTTGCGCTGTTCTTAGGTG
3' ss Score
9.58
Exon sequences
Seq C1 exon
GGAAATCTGGAGAAGATGTGCCGCACTCTGGAGGACCAGGTGAGTGAACTGAAGAGCAAGGAGGAGGAGCAGCAGCGGCTCATCAACGAGCTGACAGCGCAGAGAGCACGCCTGCAGACAGAAGCAG
Seq A exon
GTAGAGACTCCTTCCCTGGCTCCCCACTTCCTGCATTGCACAAGGGGGTTCTTCCTATGCCGTAATACTCTCATCAAATAGGGAAGTCAGGAGACTGTCAGAACCTTTTGAGGTCGACAACCAAGGTGTGGGGAAGCAAAATTAAATAGAGAAGTTTTTTATGTTCCTGCGGCACCCCTGCTTGCAAGCTACATTTCTGACTTCTTTGTTTGCTAATGTCTATTTCTATAAGAATGGTTAGAAATGAATAGAATATATACACGCACACACAGCGTATTGTTAAACCAAGAGTTCTCCCACTTGCGCTGTTCTTAG
Seq C2 exon
GTGAGTACTCCAGACAGCTGGATGAGAAAGATGCTTTAGTCTCTCAGTTGTCAAGGAGCAAGCAGGCATCCACGCAGCAGATTGAGGAGCTGAAGCGTCAGCTCGAGGAAGAAACCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000055775:ENSMUST00000019625:28
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.814 A=NA C2=0.875
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.9=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAATCTGGAGAAGATGTGCCG
R:
CTTGGTTTCTTCCTCGAGCTGA
Band lengths:
246-561
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types