MmuINT0110600 @ mm10
Intron Retention
Gene
ENSMUSG00000049134 | Nrap
Description
nebulin-related anchoring protein [Source:MGI Symbol;Acc:MGI:1098765]
Coordinates
chr19:56354120-56355586:-
Coord C1 exon
chr19:56355482-56355586
Coord A exon
chr19:56354228-56355481
Coord C2 exon
chr19:56354120-56354227
Length
1254 bp
Sequences
Splice sites
5' ss Seq
GAGGTACTG
5' ss Score
7.67
3' ss Seq
CTCCCCTAAATCTCCCCCAGACC
3' ss Score
8.68
Exon sequences
Seq C1 exon
GTGGCCTACAAGGCAGGAAACGAGCAATCCGTCCATCAGTACACCATCAGCAAAGACGAGCCTCTCTTCTTACGAGCCCGGGCCAATGCTGCTCAACTCAGCGAG
Seq A exon
GTACTGCAAGGGTGGGCACTGCCGGCTTGGCTCCCCCATGTGTCTGTGATCAGCTGGTCACCAAAGCCACCAGCTACACTGCAGTGATTGGTTCAACTGCAGTGGGTGTGGTGTGGTTCCTGACCAATGCTTGTTCCAAGAGTCAACCCCGAGTGGTCGAAGGCGGGAAACCCAGTTGTCAACTCTACACCTTGGTCCTTAGCTTGACCACTTACCAGGGGTGCAGCTATAGACTGGGCGTTTACTCTTGGATGTCCGTTAGTGAAGTGAAGCTAAGAGCAGCTCTTCCTCCCTGGGTTGCCACGAGGCTTCCGTGAGCATACATGCATGATATTCTTCAGGAGTGAGCACTGTGGTAGCTCCCTGGTGTGACATGTCCATGATTGATATCCCGTCCCAGTACGCCTGCTCTTGACTCTCTATACACAAGTGTCCCAAGTCTCTGCCGTCCTGACCTGGAAATAGGCAGGGAGGAGAAACAGAAGCCGGGGTAGAGGCTGAACTGATATCCACTCTGAGAGGAAGGGAGGCCTTCCAGAAGAAAGGCCTTCAGGCTTGGAGGCAGAGGTTTCAGATGGAAGGGACAAAGAACAGCTCTAGGACTGGAGGCTGGATGAGGGATGATTCAGGAGAAAGTCCTCCGAGCCCTGTGGCTAAGTACTCTGGCTGAGTCTTGTGGCTGAACCCTGTGGCTGAGTCTTATAACTGAGCCTGTGGCTGAATCCTATGACTGAGTTCTGTGGCCGAGGCCTGCTCCTGGTGTGTGTATGGCTGAGTCCTGTGCTAGCCCATGGGATGTCGTAGCATCTGTCATGCTTGAGAGACTTCTGTGTGGTACCTATGGTATGTTTCAGACTGATGTGAGGTTTATCCTTGCTTCTTATGGTGGAAGTCAGATAAGCCAGGAGGCCCTCAGCAAGGGTAAAGAGACCTGGTTCTCAGGCTAGGCAAGGATGACACTGCTGATGCTCAGAAAGGTTTTCACAGAGTCTAGAGCAGTGGTTTTCAACCTGTGGGGGGTTCCCAGGGGTCATATATTAGAATGTCCCACATATCAGATATTTACATTACAATTCATAACAGTAGTAAAATTACAGTCATGAAGTAGCAATGAAAACGATTTTATGGGTAGGGGGTCACCACAACATGAGGAACTATATTAAGGGGTCAGAGCATTAGGAAGATTGGGAACCACTTATTCTAGGGGAACCCTCTCACTTAAAGATAACCATGGCTCCCCTAAATCTCCCCCAG
Seq C2 exon
ACCCTGTACAAGAGCAGCTGGGAAAAGCAGAAGGCCAAAGGGTTTGAGCTGAGACTTGACTCCTTGGCATTCCTGACAGCCAAAGCCAAGAGAGACCTGGCTAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049134:ENSMUST00000073536:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.029 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTACAAGGCAGGAAACGAGC
R:
TCACTAGCCAGGTCTCTCTTGG
Band lengths:
208-1462
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types