RnoINT0103044 @ rn6
Intron Retention
Gene
ENSRNOG00000016714 | Nrap
Description
nebulin-related anchoring protein [Source:RGD Symbol;Acc:1306731]
Coordinates
chr1:277139454-277140953:-
Coord C1 exon
chr1:277140849-277140953
Coord A exon
chr1:277139562-277140848
Coord C2 exon
chr1:277139454-277139561
Length
1287 bp
Sequences
Splice sites
5' ss Seq
GAGGTATCA
5' ss Score
6.35
3' ss Seq
GCTCCCTTAAATCTCCCCAGACC
3' ss Score
7.83
Exon sequences
Seq C1 exon
GTGGCCTACAAGGCAGGAAACGAGCAGTCCGTCCATCAGTACACCATCAGCAAAGACGAACCCTTCTTCCTACGAGCCCGGGCCAATGCTGCTCAACTCAGCGAG
Seq A exon
GTATCACAAGGGGTGGGCACTGTGGGCTCCGCTGCCCTGTGTCTCTGTGGTCACCTGGCCACCAAAGCCACCAGCTACACTGCAGTGTTTGGTTCAGCTGTAGTGTATGTGGCCTGGTTCCTGACCAGTGCTTTGGGGAAGAGGAAGACAAACCTCCTAGCAATGGCTGGCAGGGTCTTGAGTTACTGACTAGACCATAGTGGGCAGAAGGGGGCTTAACCCAAGAGTCAGCACTAAGTGGTAGGAAGAGGGAAACCCAGTTGTCGACTCCAAACCTTGGTCCTTAGTTTGACCACTTGCCAGGGGTGTAACCATAGACCAGGTCTTTACTCTTGGATGTCCCTTAGTGAAATGAAGCTAGAACCAGCTCTTCCTCCCTGGGTTGGCACGAGGGCTTCGGGAAGTGTGCGGCCATGATGTTCTTCAAGAGTGTGCATTATGGTGACTCCCTGACGTGACAGTGTCCGTGGTTGACATCCCGTCCTAGTATATCTGCTCTTGACTCTCTATACACAAGTCTCCCAAGTCTCGGTCCTCCTGACTTGGGAACAGGCAGGAAGGAGAAACAGAAGACGGGGCAGAGGCTGAACTGATTTCTCCTCTGAAAGGAAGGGAGGCCTTCCCGAAGAAAGGCCCTAAAACTTGGAGGCAGAGGTTGGGGGTGGAAGGGACAAAGGACGGCCCTAGGACTGGAGGCTGGAGAGGGATGGTTCAGGAGAGAGTCCTCTGAGTCTTGTGGCTGAGCCCTGCTGATGGCATGTGTAGATGAGGTCTGCGGCTGAGCCCTGTGGCCGAGTCCTGTGCTGTCCCACGGAATGTCGTAGCATCTGACATGTTTGTGAGACTTCTGTGATGTCCTGTGGGTATGTTTTTAAAGCAATGTGAGGTTTAATCCTTGCTTCTTGTGATGGAAGTCAGATATACCAGGAGGCCCTCAGGCAGGACAGAGACCTGGCTCTCAGGCTAGGGTTGATGTTATTGCTATTGCTCCTTAGAAAGGTTTTCACAGAGTCTAGAGCAGTGGTTCTCAACCTGTGGGTCAAGACCCCTTTGGGGAGTTCACAGGGGTTGTATATAAGATGTCCTATATATCAGATATTTACATTACAATTTGTAACTAGCAAAAATGCAGTCATGAAGTAGCAATGAAAATGATTTTATGGTTGGGGGTCACCACAACATGAGGGACTATGTTAAAGGGTTGTAGCATTAGGAAGGTTGAGAACCACTTGTTCTAGAGTAACCCTCTCGCTTAAAGATATTCACGGCTCCCTTAAATCTCCCCAG
Seq C2 exon
ACCCTGTACAAGAGCAGCTGGGAAAAGCAGAAGGCAAAAGGATTTGAGCTGAGACTTGACTCCTTGACATTCCTGACAGCCAAAGCCAAGAGAGACCTGGCTAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016714:ENSRNOT00000038017:21
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.057 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTACAAGGCAGGAAACGAGC
R:
TCACTAGCCAGGTCTCTCTTGG
Band lengths:
208-1495
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]