DreINT0106216 @ danRer10
Intron Retention
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30486947-30487548:+
Coord C1 exon
chr12:30486947-30487051
Coord A exon
chr12:30487052-30487440
Coord C2 exon
chr12:30487441-30487548
Length
389 bp
Sequences
Splice sites
5' ss Seq
GAGGTATGG
5' ss Score
8.98
3' ss Seq
ATTGGGTTTCTGGTTTTTAGAAA
3' ss Score
6.89
Exon sequences
Seq C1 exon
TTGGCATACAAGGCAGGCACAGAACAGACAATGCACCAGTACACTATAAGCAAAGATGAGCCTCTCTTCAAGCAAGCCAAAGCTAACGCAGAGCTTCTCAGTGAG
Seq A exon
GTATGGAACTGAATCACATAAACTTGTACACTGTATAAAGTGATTAGTTACTTAAAAAAGCAAGTTAACCTCTTGCCCTAAATGTGAAAAGTTGACTTAACTTAAAGTGAATAAACCCGTTGTAACTTGAAAGTGTTACAGTATGTTGAATTATAATTTTTTTAATTATGCACACATTTCATTTATTTAAAAAAAGAGTAAACCCCTTGCCTTAAAATGTTTAAGTCAACTACAGTAATCCGTTCAAAAGCAACCGGTTTACTCCCTTTTTTAGTAAAGTCAACTGATCGCCTTTATAGTGGATACACTGTATTTTGTACCATTAACCTGCACAAGAACTAACAACCCACTTCTGCTTTTAATCAACACATTGGGTTTCTGGTTTTTAG
Seq C2 exon
AAAACATATAAGAGTAGTTGGGATGAACAGAGGGAGAAGGGCTTTGAGCTAGGCTTGGACACACTTTCGATTCTCTCTGCCAAAGCAACAAGAGATCTCGCCAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341:ENSDART00000124920:23
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.057 A=NA C2=0.028
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(57.1=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(35.7=27.8),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATACAAGGCAGGCACAGAA
R:
ACTGGCGAGATCTCTTGTTGC
Band lengths:
207-596
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]