BtaINT0103647 @ bosTau6
Intron Retention
Gene
ENSBTAG00000019327 | NRAP
Description
nebulin-related anchoring protein [Source:HGNC Symbol;Acc:HGNC:7988]
Coordinates
chr26:34375926-34377688:-
Coord C1 exon
chr26:34377584-34377688
Coord A exon
chr26:34376034-34377583
Coord C2 exon
chr26:34375926-34376033
Length
1550 bp
Sequences
Splice sites
5' ss Seq
GAGGTACAG
5' ss Score
6.62
3' ss Seq
CTCTTTTCTCCTACCCTTAGAAA
3' ss Score
10.3
Exon sequences
Seq C1 exon
GTGGCCTACAAGGCAGAGCACGAGCAGTCCGTCCATCAGTACAGCATTAGCAAAGACGAGCCTCTCTTCCTACAGGCCCGAGCCAACGCTGCCAATCTCAGCGAG
Seq A exon
GTACAGTCGGGGGCGGCAGGGAGGGGAGGCGTGCTGAGGGCTCTACTTTCCTGTATGTAGGCACCTGATCTGTAAAGCCCACCAGCTGTATCAGTGTGTCTGTGTCCACTGAGGCTGCCTGTGTACATCTGGTCGTTGTAAACTCCTCACCAGTATTAGTGGGCGGGAGGGGCTTCCCTGGTGGCTCAGTGGTAAAGAATCTACCTGCCAAACAGGAGATGCTGGTTCAGTCCCTGGGTCAGGAAGATTCCCTGGAGGAAGAAATGGCAACCCACTCTAGTATTCTTGCCTGGAAAATCCCAGGGACAGAGAAGCCTGGTGGGTTACAGTCTTCGAGGTTACAAAAGAGTCGGACGCCATTGAGCGATGGAGCATGCACACATGCACGCATGCAGACAACCTTTGGTAGGAATGAGCACCAGCTATACGGTGGAGTAGGAGGCAGTGGTTATTCTGGGGTTGGAGTTGAACAACGTAAGAATAGTGATAGAGAAGGGAGTGAAAAAGAGCTCAGAAATTGGCTCCAAACTCTCTGGGTTTTTAGCACTTACTGGGTATGCAACCGTGGGCAATTTCTTTGCTCTCAGCGGTCCCACTGGCAAATGAGGGCACGCTTATTTCCCTCTACACTGGGTTGCCCCGAAGTCTCAGTGAGCTGATATACGGCAAGCACAGGGAGGAGCATGCTCAGAGCGGTTATTCCTTAGGCACGAAAGCTTGCGGAGTTCCAACTTTTCTTCTCTTTCTTCTAAGGAAGGGCCTTTCTTGGCTCTTCTGCTGGTCTCCCAGGTCTTTGGGGGTCCTGATGCAGGTATTTGGGAGAGGGGGGAGAACGGGAAGGAGAAACAGAAGCTAAATTGATTCACACACAGAAATAGAGGGAGCCTTAGATGGTAAAGAATCTGCCTGCAATGCAGGAGACCTGGGTTCAATCCCTGGTTTGGGAAGATGTCTTGGAGAAGGAAATGTCTACCCACTCCAGTATTCTTGCCTGGAGAACCTCATGGACAGAGGAGCCTAGTGGGCTACAGTCCCTGGGGTTGCAAAGAATCGGACACCACTGAGCGACTTTCACTTTCCAGAAGAAAGAACTTGAAATCTGGAGGTGGGGGCAGGACAGGAAGTGGGAGGAGCAAAGGAAAGACCCACATGCACTAGAGGCTGGGCTCAGAAAGAGAAAGGGGTGCACCAGGAGCAGCTGCTGGGCTGGTGGGGTGTGCAGACAGAGGCAGGGAGCAGACTCTTCAGAAGTGCTGCCGTGTGTTGCGCGGTGTCATTCCACCTATGACCTGTCACTAGACTTCCGTGAAGACGCTTAGTATTTTCTAGGGCTTCTGGAGTTCAGTGTCTGCTTCTTTTAGCCAAATTCCTGTAAGGCAAGAGGCCAAAAGGTGGAACAAGAAGGCCTGGTTATATTCAGGCTATAGATTTCTCTGGGTCTCAGGATTTCTATGGAAGGATGTAATGGGTGTTTGCCTCCTGGAGAAGTTGTTATAGAGTCTAAGGGAAGTCCTCTCACTTCAGGATGATCTCTTTTCTCCTACCCTTAG
Seq C2 exon
AAACTGTATAAGAGCAGCTGGGAAAACCAGAAGGCGAAAGCATTTGATCTGCGTCTTGACTCCTTGGCCTTCCTGGCAGCCAAAGCCAAGAGGGACCTGGCCAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000019327:ENSBTAT00000025740:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.057 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTACAAGGCAGAGCACGAGC
R:
CACTGGCCAGGTCCCTCTT
Band lengths:
206-1756
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]