MmuINT0117012 @ mm10
Intron Retention
Gene
ENSMUSG00000053965 | Pde5a
Description
phosphodiesterase 5A, cGMP-specific [Source:MGI Symbol;Acc:MGI:2651499]
Coordinates
chr3:122817921-122823181:+
Coord C1 exon
chr3:122817921-122818067
Coord A exon
chr3:122818068-122823055
Coord C2 exon
chr3:122823056-122823181
Length
4988 bp
Sequences
Splice sites
5' ss Seq
GAGGTAGGA
5' ss Score
8.24
3' ss Seq
CTTGTGCCCATTTCCTACAGGTT
3' ss Score
10.52
Exon sequences
Seq C1 exon
GCTGCTGTGGTACCATCGGCCCAAACCCTTAAAATTACCGACTTCAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCGCTGTGTACAATTCGGATGTTCACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAG
Seq A exon
GTAGGAAAGCTCTCTCTCTCTCTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACTCACGTGTATATAAGATAGAGAATTGGATCTGACTGCTGGCACATAATAGGGATTTTCTGTTTTACTGATTAACATATCGTTGCTTAACCATCAGTCATTAAAGATACATCTTTTTTTGACTCCTGCCATGTATCAAGCACCATTTTGAAGAATGCAGGAGGATCTTACTTAAAGCGACGGAGGTAGTGCAAAATCAGGTGGCAGGAGATTCTGGGTGGAACATGGGTCAAGGGGAGGGTGGCCAGAGAAGCCTCTGGAAAGACGAACAGCAGAACAAGTAGACTGGCAAGCCATCAAAGAGCAGCGGCTGCCTCCTGGCACCAGTGGTGACTCACTGAAGCAGGGAAGTAAGGCTGTCATCACCAGGGTTGTGACTAACAATGCAGAACGGACCAGGACCATAGGTGCCTGTTGTGGGGTCAGACTCCTCAAAGGAGGTCATCTCTGTGGAGCAGAAATGAGACCCTTTTGAACCCCAGTCTCCAAATATAGATTTCCTATCTAAACATTTATAGGTCTTCAAGGTTTTCACTGCTTAGCCTTCCATTAATTCATAAAGGGAGAAGTTACTCAGTGTCCTGTGTGGTTCTGATGAGTGGACCATGCTCTCAAAGACCTCACCGTATGGAAAGAAAGCTAAGGGTAAATATAGCTAAGGTTCATAACGTGTCCTTTGTGGTCCCAGAGACCCGAGTCTAATTATTATTTTTTTATTTAACCAAAGTTTATATTATATATATTTTTTAAGATGAAAACCCAACAATACAAATAGGCTAATGATGTGTCACTCCTGCCTGGGTGTTAGTTAAGGGATATCATGTTGTCCAGTTATTCCAACAAGCTCTCATGAATGAATCTCATTCACAGATGTAGAACCCAAACCAGCGTGGATTAGAAATGTCACGGTGTCACTCATAGCTCGCTCAGTGATTAGCACAAATTCACTTTGGCAGTTTATCTCCACAGTCTCAGTTTCCACACCTCTTAGAGTAAATGATGTTGCTACTCTGTGGGCGTCCGTGTGAATTACATATCACATGGTTGTGAAATGTGTGCTTGTGCTATTCTGCTTAATCCATAGATATCGAGAGCACATCAGTTATCCCACCTTTAGACAAAATTTGAATATGCTAAATCACCATCAAATTTCTCCACTGTACAAGGTAGGACTCACTTTAAAAGTCAGCTCCGTGAGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTGACAACCATCTGTAATGGGGTCTGATGCCTTCTTCTGAGGTGTCTGAAGACAGAGACAGTGTAGTTACATACATGAAATAATAAATCATTAAAAAAAGTCTGCTTCGTGAATGTAATAAGTTCACATTTAGAGTTAGAAGCCCTACATTACATGTGGATCCCAGTTTTACTTAATACCTTGATCGGATCCATGTGTTCCTTGGAGTGTCCACATCTGTAACTAACACGGACTCTACTCCAAGGGCTTGGCTGGAACACAGGGATGTGATTTTTTTTTGGTAAAACTCTTGTTGATTTACGTTTTGTTTGGCTGATGGGCAGCATTTGAGGACTGGGTAATTCAGATACTCAGTTTCTCTAACAATCATTTCCTTGGATTCCTACCCATAGGAAAATACTCCTCACCTACTTGCCTTTCGTTTGTATGTAACTTCTATCTGAATGTAAAAGACACAGATGATAGGTAATGCAGTGTCTCTCAAAGAAACACATGAATAGAGTAGACAGGCAAGACAATTTCATTTTGCAAAGAAGTGGAGCTCTGTGGCCAGCAAGCACACCAGGGTAGGAAGTGTACTTTGGTTTTATTCTAATGCAGATGGTGGCTGTGTATTTTCCCCACTGGCCAGTTTCTGGCCTCACTGTCTTTGAGACTGGTTAGTTTTAAAAGAGTCAGTGCTAAGAAAATGAAGGAAAGGGACGGGCTGCTCCTGGCCATAGAGTTCCAGGAACACAGGAAGCCCTTAGCATAAACAAAAGCTTTACCAGGTGGATGGGATTAATAGATTTGCAGGAAAATTGCACAAGACAGGGAGTGAGGGTGGGGATGGGGATGGGGTAGGGGTAAAAGATTTTAATTCCTTTACGTCAAACTTTAACAAGTTGAAGGAATTTTATGGAATATTGGCACTCTGTGGTCTAAGGAACTTAGATGAAAACAAAACAGAACAAAACCACGTTTGTTTATATTTACTCTAGGCAACGTCAACCTGGGCATTAGGATTATTAGGATTGCATTTCCTGTGCTCTGTCTAGTCGTCTCTTTTATTTCAGGTTTTATTGTCAATATAATTTTAGATGGAGAAAATTAAAATTGCTTGCTAGAGGGGATGCCCCTACCAAGACATCTCACACCGAACAACAAACCTTCGCTATTAATTTGTGCTGATGGCAGCCATTGCCTGTTTAGTGGTCTCTGAAACAATCCACTTTATTAGGACAGCACTTGTTGATTGTATTCCCACTTCTGCCCTTTCTGTCTCTGCCCCCCACCCCCACCCCTGAGCTGCCCTTGCTTGCTGCACCTCAGCCACTGTCCCTGGGGGACAGGTCCTTTCTGTAGTTTCTTAGTCACGGAGGAAAAGAATTTGTAAACAGATGTAGAAACTTGAGGACCCTTTTATTGGAGTTTGAGAGATATCGCAGGGATGGTAAGTTGTGAGTCTCAGCAGCTGCCAGGAACAGAGGGAGCGAAACGTCAGGCTTTGTGAGTGGGAGGTCCAGAGCCCACACAGGCTCAGCAAGTCTGTGAGCAGCTGCTGGGGTTGGGGGTTTTTCAGAAAAAGAAAAATGCCCAGTGACAGGGCAAGCAGGTTTAAGGTTTGGGTCAGTTTGGTTCTGAAAGAAGAGATATGAAGAGGGGAAAGAAAATGTTATTCTCCTTGGATTAGAACTAGGCAGGCTTCACAGTGAGTTCGTGATTAGTGCACCCAGGACTACAGCTTCTGAGGTCTGAGTCTTCTCCTTTCTTAACTTGTGCTTAGGCGCTTCATCTTCCCACCTCCTGGGAGCAGTTGTACTCTTAAGACTGTCTTTAAGACTGTCCCTTATCCCAGGCAGTCTTTGGCCTTCCTCAAAGGTGTTATCAACTACCGACCAAGCCTCTTTACTGAGAATTAACCTGAGCCCTGCTTCAGGTTCTAAGCAGGAGTTATTGTGACAGTTCTCCCTCCCCCAGAGACTCAGGAAGTGGGGTGCAATGGATGCTGGGATGGAGGGGACCATCCTCAAAAGGCTCCAAGCCTTATATGTTTTATCAAATTCCAAACAAGAGTTAGGAATGACTTTAGCCATTGATTCAATTGCAGGGTATGTATACACAATTCAATAGCAGCAGCCTTACGCTTTTGAACTAGTGAAAGATGTACGTAGACACCAAATTTGCAGTCTCCCTGATGATTTTCTTGCTGCTTTCATTAATCACAAGTAGTTTCCTATATGATGCCAATGCATACTCTCAAAACACATTAAAGTAAAACTTGGGGCTTTCATTTAAAATCCTCCTGCTGTAGCTGATCTGTTATCTCCCCACCGGACTGTTCAGAAATATCCAGAGGAAGGAGATGGGTACTGAGATGTCAAGGCTCAGGGAGAACACACCCGCCGCAGGCACACCAATGTACTTAACATAGGCAGCTCCCAGGGCCCAAGTCCAGAGGTCCCAAGGTTCTTTTGGGGGAGAATTCCCTGTGAGCTGAACTGAGAACTAGAAGAGAAGGTCATCTGGAAAAAAGGGATCATGCCAAGTCCCCCGTGACTGAGCAGTTTCTACCAACAGGGAGGAGGTTCCCGCTCAGCCCCCAGAGCCAATCACAAGGATGGACACTTGCCATGCTTTTCTCATATACTTTTTCCAACCCCAAGGAAATGCTCTTTGAATTTGCAACATCCCCACAGACACACTCAGGAAAGCCCGTGCTTGTTTCTCCAAAACTCGTAGTAAATCATTCAGATTTTGAGGAGAAGCAGACTGCTAACACAAGCAGAACCATCATGCTCAGATCACGAGTCCCTGAGGGTTTTATTTAATAGCCCCCTTAGAAGTTAAATTAAACCCAACTTGGAAGACTTGCTTGAACAATTTTGCCTTTTTCCTGCTTCCCATGTTTTTTTTTTTTTTTTCCTTATGCGTCCCAGGAGGTTTATACAGTATCTTCAAATTTTGATTTCTTGGTAGCAAGGCATTAAGTGCATTAGGCAGAACCTAAGCCATTAAAGATCTAATGTAGAAGGCATTAAAATCTGTAATTCTAGTTTGTCCTGAGACGTGAGCTAGATACCTCAACCCCAGCCCAGCTTAGATCTGAGCATGGTGGCTCTGCTTGTGTTAGCAGTCTGCTTCTCCTCAAAATCTGAATGATTTACTACGAGTTTTGGAGAAACAAGCACGGGCTTTCCTGAGTGTGTCCGTGGGGGATGTTGCAAATTCAAAGTGCACCACACGAGAACCTGAGAGAACTCATTTCTGAACATCCATGCACATATGACTCTGGATTCTAATGATGACATGGGAGAATTTTTTTTATAAAGGTAGTTGGTGTCCTACCTATGACAAAGTACCTTTCTTTGAGATATTACCTATTTTGTTATCTAACTTAACTAAGTCTACTTGGTAGGTTTTGGTTGAACTGGTAATGCTTGAATTCTGGCCCCGCCCCTTATGAACAAACACCAATCACAGGTTGTCTGTTTGAGAGTTGGCTCCTTCCTCTGTAAAGTGATCAAATAGTCATGTGTGCCTCCCTCACACTTGTAGGGATTGAATGTGAACACTGCGGGGTACCTGGAACGTCGAAAATGTGTAACTAACTGGGCAGTTACTGTCACTCCTTGTGCCCATTTCCTACAG
Seq C2 exon
GTTCTTTGCAGGTGGATTTTAAGTGTGAAGAAGAATTATCGGAAGAACGTTGCCTACCACAATTGGAGGCATGCCTTTAACACGGCACAGTGCATGTTTGCTGCTCTGAAAGCCGGCAAGATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053965:ENSMUST00000066728:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0023314=PDEase_I=PU(26.4=54.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types