MmuINT0117061 @ mm10
Intron Retention
Gene
ENSMUSG00000029491 | Pde6b
Description
phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide [Source:MGI Symbol;Acc:MGI:97525]
Coordinates
chr5:108403320-108404167:+
Coord C1 exon
chr5:108403320-108403472
Coord A exon
chr5:108403473-108404077
Coord C2 exon
chr5:108404078-108404167
Length
605 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGT
5' ss Score
7.77
3' ss Seq
TCTTATAATTGCTGTTTCAGGTT
3' ss Score
9.92
Exon sequences
Seq C1 exon
TGTCCCCACTTCAGCTCATTCGCCGATGAGCTCACCGACTATGTGACAAAGAACATTTTGTCCACACCAATCATGAATGGCAAAGATGTCGTGGCTGTGATCATGGCAGTGAATAAACTGGATGGCCCATGCTTCACGAGTGAAGATGAAGAT
Seq A exon
GTGAGTGTCCAGGAGTTGTTAAACACTCAGCTCTCCCCATGGGTTTGCCCATGTGTGTGTCTGCGTTTGTCCCAGCATGCCTTTGCTCATCTGAATGCCGTTATCTCTCTTTCATGTGGTTAAGTGGGCCATGCCCCTTGACAAGTCTAGGTCTGAGTTGTGTCCCTCTGTTAATCTGAATGTGTGAGCTGTGCTTATCCAGAGGCGTGAGTCACACTCTCACAGAGATTCTTCAAATCTCTCTGTGTCTCTATTTATCTCTGTGTGTGAGCTATGCCCCAGCTGTTCATCTGTGTGTATGTAAGTCATGTCCCTTTGTGTGTGATCCATGTCCTTGCCCCACAGGACTGAACCTTTGTGTGCAAGTCCACACCCATGAGTACTAAGTATAGTCTACCAGTATTCTTGCGTGTGTGTGATACTTCCTCCAGACCTTTGGAGTATGCATCTCTTCTGTACATGTGTCCTTAAACATCTGGATTTCTGGTATGCCTCCCTTCTATGCATGTGTCCTTGAACATCTGGATTTCTGGTATGCTTTTTTCTATACATATGTCCTTGAACATCTGGATTTCTGACTCATGCTCTTATAATTGCTGTTTCAG
Seq C2 exon
GTTTTCACGAAGTACCTGAATTTTGCTACATTAAACCTGAAGATCTATCACCTAAGCTACCTCCACAACTGTGAGACACGCAGAGGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000029491:ENSMUST00000031456:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=FE(33.3=100)
A:
NA
C2:
PF0159021=GAF=PD(8.0=40.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCCCACTTCAGCTCATTCG
R:
CCTCTGCGTGTCTCACAGTTG
Band lengths:
238-843
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types