MmuINT0119703 @ mm10
Intron Retention
Gene
ENSMUSG00000026447 | Pik3c2b
Description
phosphoinositide-3-kinase, class 2, beta polypeptide [Source:MGI Symbol;Acc:MGI:2685045]
Coordinates
chr1:133078219-133079839:+
Coord C1 exon
chr1:133078219-133078252
Coord A exon
chr1:133078253-133079643
Coord C2 exon
chr1:133079644-133079839
Length
1391 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
3' ss Seq
CTGACCTCCCCACTTCACAGAGA
3' ss Score
8.34
Exon sequences
Seq C1 exon
GATCCCAGCGTGTTGAGTGTGAGGGAAAACCGAG
Seq A exon
GTAAGGATGCTGCAGAGAGGCTGGAACCTGCCAGCTGCCAACTCAGCCCACATTCTTCTTCCTTCTTGGCCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTCCATATGAGGTGTGGACACAGCTGGAGCCCATATTTCTTTCCACAGATCTTTTTCTCATTATTCTTGCTAATCAGGCTTATGGTGTTACTGGGAGAGAGCGGTGAGCTGGGGTAGGGGTGTGAGCAGGGATGAGTTTGTCTGTCAATCCAGATGGAGAAGCTCACCCTGCTAGGCTGTGATTTTTTTCTTGGCAGCCACAACCCCTTGCACTTCCAGTTAAGACTGGGCCTTGGGAGATGGGAGGAAGGATTCTCTCTAGCAGTCTTCCCATCAAATCTTGAACAGATCCCCATCCTACAGCCTGTGTCTCCAGCTTTTCTTTTCTTTTTTTCTTTCAAATTTTATTTTTTTGTATGTGCATTGTTGTTTTGTCTGCTTGTATTCCTATCTATGTGGGGATGTTGGATCCTGGTGTCAGTTGAGAGATTCCATGTGGGTGCTAGGAATTGAACCTGGGTCCTTTGGAAGAACAGCCGGTGATCTTAACCACTGAGCTATCTCTCCAGCCCCATCACTGGCTTTCTTAAAAAATGAATCCATCTTTGGACTTCAACAGATGAGTAAATTTCCTCTAAAGTCGTCTAGGAGCTAACAGCATTTCTTCTAGCGCTCTTCTCTCTCTCTCTCTCTCCCTCTCCCTCTCCCTCTCCCCATCTCTCTCTCTCTCTCTCTCTGCCTGTGTATGTGTGTGTCTGTGTGTCCACATGTGCATGGTACTAGAGATGTGTAAGCTCACCAAGCACTGTTTGTTTGTTTGTTTTTGTCAAGAAAAGTCTTTGTTTAAAAAAAAGATTTGTTTTTATTTTATGTGTATGGGTATTTTGCCTATATATAAGTCTGCGTGGCACATGTGTGCCTGATACCCACAAAAATCAGAAGAGGATGTTGAACCCCTGCCCCTGGAACTGGAGTTAGAGTTGTGAGCTGCCATGTGGGTGCTGGGAACCAAACCTGAGTCCTCTGGAAGAGCAGCTAAGTGCTCTTAACCACTGAGCTGTCTCCCTAGAAAATGACTTTTTAAAAAATAACAATGTTGTAGCACTAAATGATGCTAAGTGTCTGGATAAGGCCCGAATTTAAAACTGGGCACTCAGCTTAATGCACGCTCCTGTATCTTTCCCATCTCTCCACACATGGGTAAGCTTTGTCACAGCTATTAGAAATTGGCTGATCCCTGTCCCAAGCTGTCCCATACTGCTAGTGTCATGGCAACCAAATGGCCCCTATTGAGTCCTAATGAGATTCTTTTCTGACCTCCCCACTTCACAG
Seq C2 exon
AGAAGGTGGTAGAAGCCTTGACAGCGGCCATCTTGGACTTGGTGGAGCTGTACTGCAGCACATTCAATGCAGACTTCCAGACGGCGGTGCCTGGGAGCCGAAAGCATGATTTGGTTCAAGAGGCCTGCCACTTCCCTGGGGCCCTGGCCTTCACTGTCTATGGCACTCACCGTATTCCCATCATCTGGGCTACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026447:ENSMUST00000077730:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.250 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0079219=PI3K_C2=PU(0.1=0.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATCCCAGCGTGTTGAGTGTG
R:
CCAGATGATGGGAATACGGTGA
Band lengths:
222-1613
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types