MmuINT0131995 @ mm10
Intron Retention
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136102235-136102665:+
Coord C1 exon
chr5:136102235-136102427
Coord A exon
chr5:136102428-136102527
Coord C2 exon
chr5:136102528-136102665
Length
100 bp
Sequences
Splice sites
5' ss Seq
CAGGTACTC
5' ss Score
7.04
3' ss Seq
TCTACTCTCTGCCCTTCCAGAAC
3' ss Score
10.55
Exon sequences
Seq C1 exon
GTGCTCTGGGCTGCACCGCCCACAGACCGAAGCTGAAGTGTTAGAGCAGAGTGCACAGACGCTGCGTGCCCACTTAGTGGCGCTGCTGAGCGCCATATGCCGCTCGGTTCGCACGTGCCCAGCCATCATCCGCGCCACATTCCGGCAGCTGTTCAGGCGCGTGCGCGAGCGCTTCCCGAACGCCCAGCACCAG
Seq A exon
GTACTCCCCGCCCTGTCAGCAAAGCTAAAGGCCGGGACAGAAAGGGAATCCTGCGGGACAGATATGCTTCTTGACCAGGCTCTACTCTCTGCCCTTCCAG
Seq C2 exon
AACGTACCATTCATCGCGGTCACCAGCTTCCTGTGCCTGCGCTTCTTCTCTCCCGCCATCCTGTCGCCCAAGCTCTTCCACCTGCGAGAGCGGCATGCAGATGCCCGCACCAGCCGCACGCTGCTTCTGCTGGCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952:ENSMUST00000042135:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.015 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(33.7=100)
A:
NA
C2:
PF0061614=RasGAP=FE(23.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCACAGACCGAAGCTGAAGTG
R:
GAAGAGCTTGGGCGACAGGAT
Band lengths:
252-352
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types