MmuINT0132010 @ mm9
Intron Retention
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136576772-136577264:+
Coord C1 exon
chr5:136576772-136576889
Coord A exon
chr5:136576890-136577113
Coord C2 exon
chr5:136577114-136577264
Length
224 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
3' ss Seq
AGCATTCCCCCTTTCTGCAGGGC
3' ss Score
9.19
Exon sequences
Seq C1 exon
GGGCAACCTGGGCTCCTTGCAGTTGGAAGTGCGGCTGCGGGACGAGACAGTGCTGCCGTCCGTCTGCTACCAGCCCCTGGTACAACTGTTGTGCCAGGAGGTGAAGCTGGGCACTCAG
Seq A exon
GTGAGGAGCCCCTGAGACTGGGAGCAGCTGTGGTTAGATGAACAGTTCACCTGTCCCGAACCCATTCTACAGAGCAATAAACTGATCCCCGGGTCTGAGAGGGCACCAACCAATGTCCAAAGGGCCCCACTCTCTGCCCAGGCAACCTTCCCATGATGCTCTGAGGTTTTAACCCTGGGGAGCTTTAAGTTCTCTGTGGGTCTGAGCATTCCCCCTTTCTGCAG
Seq C2 exon
GGCCCAGGACGGCTGATTCCTGTCATTGAGGAGACAACTAGTGCCGAGTGTCGCCAGGAAGTGGCCACCACCCTGCTCAAACTCTTCCTGGGGCAGGGGCTGGCCAAGGACTTTCTGGATCTGCTTTTTCAGCTGGAGCTGGGCCGCACCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952-Rasa4:NM_133914:9
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.025 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0061614=RasGAP=PU(6.8=25.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Human
(hg38)
No conservation detected
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCTGGGCTCCTTGCAGTT
R:
TGAAAAAGCAGATCCAGAAAGTCCT
Band lengths:
245-469
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: