MmuINT0132064 @ mm10
Intron Retention
Gene
ENSMUSG00000052142 | Rasal3
Description
RAS protein activator like 3 [Source:MGI Symbol;Acc:MGI:2444128]
Coordinates
chr17:32395075-32395966:-
Coord C1 exon
chr17:32395822-32395966
Coord A exon
chr17:32395220-32395821
Coord C2 exon
chr17:32395075-32395219
Length
602 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAAC
5' ss Score
3.32
3' ss Seq
CTGTCTGTGCTGGACTGCAGGGC
3' ss Score
7.34
Exon sequences
Seq C1 exon
GCATTGGTGACAGACCTAGGAACCGCAGAGCTGGCACGCTGTGGAGGCCGTGAAGCACTGCTATTCCGAGAAAATACCTTAGCCACAAAGGCCATTGATGAATACATGAAACTGGTGGCACAGGAGTACCTCCAGGACACCCTGG
Seq A exon
GTAAACAAAGAGAGGGATTGGGCCAGGAAGCCCCCAGTGAAACTGCCTTCTGGGGGTGAATGGCGCTTTGGGGTACCAGAATCTGGAGTGGGAATGGGGTTGCTATTTCCTCAAGATGTTTGGTGTTTCCAGAGTAAGTTGGAGATAGGCTTTATCTTAGGGGTTTTATTGCTGCAAATGAAACACCATTACCAAAAAGCAAGTTGGAGAGAAAAGGGCTTAGCTTTCACTTCCACGCTGCTGCTCATTATTGAAGGACGTCAGGAATTCAAACAGAGGAGAATCCTGAAGGCAAAAGCTGATGCAGACATGGAAAGGTACTGCTTACATGGCTTGCTCAGTCTGCCTTCTTATAGAACCCAGGACCACCAGCCTGGGAAAACACCACCCACAATGGAATGGGACCACCCCCACTGATAACTAATTGAGAAAATGCCTTAACAGCTGGATCTCATGTAGGCATTTCCTCAACCAAGACTCCTTCCTCACTGATGACTCTAGCGTGTGTCAAGTTGACACACATAACCAGCCAGTACACAGTTGGAGGTTCATGGAGTACATGTTTGGGTTCATGACCTGAGACTGTCTGTGCTGGACTGCAG
Seq C2 exon
GGCAGGTTGTGCGGTGTCTCTGTGCCTCCACTGAGGACTGTGAAGTGGACCCCAGCAAGTGCCCAACACCAGAGCTGCCAAAACACCAAGCTAGACTGCGAGACAGCTGTGAGGAGGTTTTTGAAAACATCATCCATTCTTACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000052142:ENSMUST00000063824:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.110
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PU(27.3=95.9)
A:
NA
C2:
PF0061614=RasGAP=FE(27.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCATTGGTGACAGACCTAGGA
R:
AAGAATGGATGATGTTTTCAAAAACC
Band lengths:
286-888
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types