MmuINT0140059 @ mm10
Intron Retention
Gene
ENSMUSG00000038677 | Scube3
Description
signal peptide, CUB domain, EGF-like 3 [Source:MGI Symbol;Acc:MGI:3045253]
Coordinates
chr17:28162954-28163857:+
Coord C1 exon
chr17:28162954-28163070
Coord A exon
chr17:28163071-28163731
Coord C2 exon
chr17:28163732-28163857
Length
661 bp
Sequences
Splice sites
5' ss Seq
GGGGTAAGC
5' ss Score
7.79
3' ss Seq
ATCTCTGACTCCTCCCACAGATG
3' ss Score
10.93
Exon sequences
Seq C1 exon
ATATAGATGAGTGCTCCTTCGACCGAACCTGTGACCACATGTGTGTCAACACCCCGGGGAGCTTCCAGTGTCTCTGTCATCGTGGCTACCTTCTATATGGTGTCACTCACTGCGGGG
Seq A exon
GTAAGCCAGCAAGATCACACCTACACCTGCCTGCTTCCCAGACACCCCAGCCCTCGACTCTTCACACTCCAAATGGCTGTGCTTGTAATGAGAAATCCTTGGATGGTTCTTGTCTACATCCAACAAGATAAAATAGAAGGCACCTTCCAAAGACCAGCTGGGAGACAGGATACCAGAGAGCAGCATCCTGGCAGCAAGATGCTTGAGGGTTTCACGTAGCCCAGGATAGCTGCACACACACACACACACACACACACACACACACACACACACACACAGCCAGTGGTGACCTCGAACTCCAAACCTCCAGCCTTCACTTCTCGAGGGTTGCGATGGCAGGTGGCACAGACTCTGCCTCCTAATTCTACCTTGCATTCCCCATCGCTGTTCCCAGAGACCCACCCTCTCAGTCTCTAGCCCAATGAGACAGTAAGAGAGGAAAGTTCTGAAACCTTGGTCTGGTTGGATTGGCCCCTGCTCAATGGCAACTATGACTGGGGATGTAGAGAAGGGAGAGAACTTTGAGCTCCTCACAGGCCAGAGAACCTTTTACTTTGTTTATGAGAATGTTAACGTGAGACCTACCTTCCTGACAAGATTTGTAAATGCACAGTAATCTCTATAAGGGTACTGGCAATCTGATCTCTGACTCCTCCCACAG
Seq C2 exon
ATGTGGACGAATGTAGCATCAATAAGGGAGGCTGCCGCTTTGGCTGCATCAACACCCCAGGCAGCTATCAGTGTACCTGCCCAGCAGGCCAGGGCCGCCTTCACTGGAACGGGAAGGATTGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000038677:ENSMUST00000043503:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=95.0),PF0764510=EGF_CA=PU(0.1=0.0)
A:
NA
C2:
PF0764510=EGF_CA=PU(89.4=97.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGAGTGCTCCTTCGACCGA
R:
CTGTGCAATCCTTCCCGTTCC
Band lengths:
238-899
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types