MmuINT0141138 @ mm10
Intron Retention
Gene
ENSMUSG00000026121 | Sema4c
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C [Source:MGI Symbol;Acc:MGI:109252]
Coordinates
chr1:36553224-36553567:-
Coord C1 exon
chr1:36553471-36553567
Coord A exon
chr1:36553341-36553470
Coord C2 exon
chr1:36553224-36553340
Length
130 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGT
5' ss Score
8.73
3' ss Seq
ACCGCTGTTGTCTCCCGCAGACG
3' ss Score
13
Exon sequences
Seq C1 exon
AACATGCTCACGTTCACCTTGGACCGTGCAGAATTTGAGGATGGGAAGGGTAAATGCCCATATGACCCAGCTAAGGGTCACACCGGACTCCTTGTGG
Seq A exon
GTGAGTGGCTGCTGCCAGCACAGAGGGGAGAATCTGGGCCTCTGTCAGTCACGCTCTGAGTACTTACTTGTCTTCAGAGCAGTCTCTACTTTAGTTCCCTGGCCTCTCTGACCGCTGTTGTCTCCCGCAG
Seq C2 exon
ACGGTGAGCTGTACTCAGCCACACTCAATAACTTCCTGGGCACAGAGCCGGTTATCCTTCGATACATGGGGACCCACCACTCCATCAAGACAGAGTACCTGGCTTTTTGGCTGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026121:ENSMUST00000114991:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(7.5=100)
A:
NA
C2:
PF0140314=Sema=FE(9.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACATGCTCACGTTCACCTTGG
R:
TCAGCCAAAAAGCCAGGTACT
Band lengths:
210-340
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types