MmuINT0151686 @ mm10
Intron Retention
Gene
ENSMUSG00000026532 | Spta1
Description
spectrin alpha, erythrocytic 1 [Source:MGI Symbol;Acc:MGI:98385]
Coordinates
chr1:174245139-174245793:+
Coord C1 exon
chr1:174245139-174245192
Coord A exon
chr1:174245193-174245646
Coord C2 exon
chr1:174245647-174245793
Length
454 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
3' ss Seq
AAAATGTGTTTGGCCTTTAGACA
3' ss Score
5.52
Exon sequences
Seq C1 exon
AGACACCATAGGTGTGAGTGAGGAGACATTGAAAGAATTTAGCACCACATACAA
Seq A exon
GTGAGTCTAACTTCACAGTTCGCTTTTGGAAAATTCAGAATAAGTTTACAAATAATAACTTCTCTCTCCCTCTCTATGTGCCTTGATGGGATCTTCAGTGACGATCTAGTCTATGTATGTTAATTAGCATCAGTATTGGATTTTCTAGCCAATTTAATTTTCTGTTAGATCCCTGTATTTTAAAAGTAAAACATGTCTTTCAATCATCATTCAACTGGTGATTGTTTGTTCTGTGTATTTCAGCTGCTTTGGAGAACAGAATGAGTAAGAAGAATTGTCAGTGTTTTGTGTTATTCCTACTGTTACTGGGTTTAACAAAAACACTCAGAGAAACAGACTTGATTCTGTGTGTTCCCGTAATATTATAGCATGTCACTGAAGAACTAGGAATTTGATCTTTTCTGTTTTTACCTGGTTTTCTCTTTAGCTTGATAAAAATGTGTTTGGCCTTTAG
Seq C2 exon
ACACTTCGATGAGAACTTGACAGGGCGATTGACTCACAAAGAGTTCCGCTCTTGCCTGAGAGGACTCAATTACTATCTGCCTATGGTAGAGGAAGGTGAACCTGAGCCCAAGTTTGAGAAATTCCTGAATGCTGTGGATCCAGGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026532:ENSMUST00000027817:49
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.090
Domain overlap (PFAM):
C1:
PF134991=EF-hand_7=FE(22.0=100)
A:
NA
C2:
PF134991=EF-hand_7=FE(59.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTGTGAGTGAGGAGACATTGA
R:
TCCCTGGATCCACAGCATTCA
Band lengths:
191-645
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types