MmuINT1021842 @ mm10
Intron Retention
Gene
ENSMUSG00000030149 | Klrk1
Description
killer cell lectin-like receptor subfamily K, member 1 [Source:MGI Symbol;Acc:MGI:1196250]
Coordinates
chr6:129616297-129616773:-
Coord C1 exon
chr6:129616669-129616773
Coord A exon
chr6:129616333-129616668
Coord C2 exon
chr6:129616297-129616332
Length
336 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAG
5' ss Score
8.68
3' ss Seq
AGTTATAATTATCTTGACAGTAT
3' ss Score
3.9
Exon sequences
Seq C1 exon
TATCTCCAATGTTCGTTGTTCGAGTCCTTGCTATAGCCTTGGCAATTCGATTCACCCTTAACACATTGATGTGGCTTGCCATTTTCAAAGAGACGTTTCAGCCAG
Seq A exon
GTACAGATATTTGCTGCATAAACCTACAATCTGAATTCCATTCCCAAAAACCAAAGAAATGTGGAAGGAAGAACTGACTCCACAAAATGGTTCCCTGGCCTCCATGTTCACTCCCTGGTACATGGGTACCCTCATATGCATGCCTACATGCAACATGTATGTGGGCACACACACACATAGAGTTTAAAAAGTGAAAAAATATATAAAAGCAAAGAAACATTCAGTAGTGAGAGTGAAAATGTTTTAAACCATAGCTTGGCTGTTCTACAAACATCCAAGTCACATTTGTTTACAGTAGAAGTTACTAAAGTTTAACAGTTATAATTATCTTGACAG
Seq C2 exon
TATTGTGCAACAAGGAAGTCCCAGTTTCCTCAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030149:ENSMUST00000032252:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 1 annotated functions for this event
PMID: 25092887
NKG2D-FL overexpression increases T cell cytotoxicity while NKG2D-TR overexpression decreases surface expression of NKG2D-FL and reduces cytotoxicity. NKG2D-TR knockdown increases T cell cytotoxic activity. Isoforms differ in their subcellular localization.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types