RnoEX0053025 @ rn6
Exon Skipping
Gene
ENSRNOG00000002487 | Mbnl3
Description
muscleblind-like splicing regulator 3 [Source:RGD Symbol;Acc:1565834]
Coordinates
chrX:138388573-138392946:-
Coord C1 exon
chrX:138392760-138392946
Coord A exon
chrX:138390860-138390895
Coord C2 exon
chrX:138388573-138388667
Length
36 bp
Sequences
Splice sites
3' ss Seq
CTTATCTGCATGGTGTACAGGGC
3' ss Score
4.85
5' ss Seq
TTGGTAGGT
5' ss Score
7.03
Exon sequences
Seq C1 exon
GCCCTGCCGCCTGGTGCACTTCAGCTGATACCAAAGAGGTCAGCCCTTGACAAGGTCAATGGTGCCACTCCAGTCTTTAACCCCAGTGTTTTCCACTGCCAACAGGCTCTGGCTAACATGCAGATTCCGCAGCCGGCTTTCATCCCGACAGGTGAGGGCACAGCTCTGGGAATATTGGGTTTAACAG
Seq A exon
GGCCAATACTGTGCATGACTCCTGCTTCAACTTTTG
Seq C2 exon
TGCCCATGATGCACGGTGCTACACCTTCCACCATGTCTGCAGCAACACCACCTGCCGGCAACGTTCCCTACGTTCCAACAACTACAGGCAATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000002487-'10-9,'10-8,11-9
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (disopred):
C1=NA A=NA C2=NA
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSRNOT00000043258fB8467
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAACATGCAGATTCCGCAGCC
R:
TTGCTGCAGACATGGTGGAAG
Band lengths:
119-155
Functional annotations
There are 2 annotated functions for this event
PMID: 21454535
Inferred from MBNL1 ortholog: The exon 7 region enhances MBNL1-MBNL1 dimerization properties
PMID: 27733504
The presence of alternative ex.54nt always significantly elevated MBNL1 and MBNL2 protein levels (_2.7 and _4.0-times, respectively). There were marginal changes of the GFP-MBNL level for constructs carrying ex.36nt (_1.3-times) and ex.95nt (_0.8-times). There were no significant differences between the activities of the analyzed proteins for the majority (67%) of AS events. However, these exons might have either a positive or negative effect on some specific AS events (Figure 5A and C). Interestingly, exOFF events predominated in AS events with a negative effect of ex.36nt (95%; P = 0.002) and a positive effect of ex.95nt (90%; P = 0.010). All together, these data indicate that the presence of sequences encoded by these alternative exons can significantly modulate MBNL splicing activity, but this regulation depends strongly on the targeted RNA.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]