RnoEX6016451 @ rn6
Exon Skipping
Gene
ENSRNOG00000013442 | Ciz1
Description
CDKN1A interacting zinc finger protein 1 [Source:RGD Symbol;Acc:1305336]
Coordinates
chr3:11393132-11396393:+
Coord C1 exon
chr3:11393132-11393247
Coord A exon
chr3:11394659-11394730
Coord C2 exon
chr3:11396164-11396393
Length
72 bp
Sequences
Splice sites
3' ss Seq
TGCCAAATTACTTTCTTAAGGAC
3' ss Score
4.15
5' ss Seq
TGGGTACTC
5' ss Score
1.92
Exon sequences
Seq C1 exon
GGGCCTCCCCCAGCAGTCATCCCCGCAACAGCTTCTGAATCTCCAGGGCCTCAACTCGACCTCCCTGCTCAATGGCTCCATGCTGCAAAGAGCTTTGCTCCTACAGCAGTTGCAAG
Seq A exon
GACTGGACCAGTTTGCAATGCCACCAGCCACGTATGACAGTGCCAGCCTCACCATGCCTACGGCAGCCTTGG
Seq C2 exon
GCAACCTCCGTGCTTACAATGTGACAGCCCCAAACCTAGCAGCTCCTAGCCTTACACCACCCCAGATGGTCACCCCAAATCTGCAGCAGTTCTTTCCCCAGGCTACTCGACAGTCCCTGCTGGGGCCTCCTCCTGTTGGGATCCCAATAAACCCTTCTCAGCATAACCACTCAGGGAGGAACTCCCAGAAGCAGCCCAGAACCCCCTCTTCCACCACCCCCAATCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013442-'3-5,'3-3,4-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.375 A=0.760 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACTCGACCTCCCTGCTCAAT
R:
GTAGCCTGGGGAAAGAACTGC
Band lengths:
170-242
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]