Special

MmuEX0011463 @ mm9

Exon Skipping

Gene
ENSMUSG00000039205 | Ciz1
Description
CDKN1A interacting zinc finger protein 1 [Source:MGI Symbol;Acc:MGI:1920234]
Coordinates
chr2:32219739-32223052:+
Coord C1 exon
chr2:32219739-32219854
Coord A exon
chr2:32221323-32221394
Coord C2 exon
chr2:32222823-32223052
Length
72 bp
Sequences
Splice sites
3' ss Seq
TACCAAATTACTTTCCTAAGGAC
3' ss Score
3.57
5' ss Seq
TGGGTACTC
5' ss Score
1.92
Exon sequences
Seq C1 exon
GGGCCTCCCCCAGCAGTCATCCCCGCAACAGCTTCTGAGTCTCCAGGGCCTCCACTCGACCTCCCTGCTCAATGGCCCCATGCTGCAAAGAGCTTTGCTCCTACAGCAGTTGCAAG
Seq A exon
GACTGGACCAGTTTGCAATGCCACCAGCCACGTATGACGGTGCCAGCCTCACCATGCCTACGGCAACACTGG
Seq C2 exon
GTAACCTCCGTGCTTTCAATGTGACAGCCCCAAGCCTAGCAGCTCCCAGCCTTACACCACCCCAGATGGTCACCCCAAATCTGCAGCAGTTCTTTCCCCAGGCTACTCGACAGTCTCTGCTGGGGCCTCCTCCTGTTGGGGTCCCAATAAACCCTTCTCAGCTCAACCACTCAGGGAGGAACACCCAGAAACAGGCCAGAACCCCCTCTTCCACCACCCCCAATCGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000039205_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.261 A=0.751 C2=0.975
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000048428





     
                   









    
Main Skipping Isoform:
ENSMUSP00000108957





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000048428f3938A, ENSMUSP00000048974, ENSMUSP00000108958, ENSMUSP00000108960, ENSMUSP00000108962, ENSMUSP00000108964, ENSMUSP00000116812, ENSMUSP00000120295
          









    
Other Skipping Isoforms:
ENSMUSP00000121650
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr9:128187863-128187934 
ALTERNATIVE
HsaEX0015444
(CIZ1)
Human
(hg19)
chr9:130950142-130950213 
ALTERNATIVE
HsaEX0015444
(CIZ1)
Rat
(rn6)
chr3:11394659-11394730 
ALTERNATIVE
RnoEX6016451
(Ciz1)
Cow
(bosTau6)
chr11:98821351-98821422 
ALTERNATIVE
BtaEX0009092
(CIZ1)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ACAGCTTCTGAGTCTCCAGGG

				
R: 
AGAACTGCTGCAGATTTGGGG

				
Band lengths: 
182-254

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 1 annotated functions for this event 


PMID: 32249828
HsaEX0015444
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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