RnoEX6040609 @ rn6
Exon Skipping
Gene
ENSRNOG00000014851 | Col4a4
Description
collagen type IV alpha 4 chain [Source:RGD Symbol;Acc:1305355]
Coordinates
chr9:88272130-88274271:-
Coord C1 exon
chr9:88274170-88274271
Coord A exon
chr9:88273162-88273343
Coord C2 exon
chr9:88272130-88272193
Length
182 bp
Sequences
Splice sites
3' ss Seq
TATCACACTGACTTCAACAGGTG
3' ss Score
7.73
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
Exon sequences
Seq C1 exon
ACAGGGGACCTCCTGGTGATGTAGGAGAAACAGCCGTTTCTAGAAAGGGGGAACCTGGGGATGCTGGGCCTCCAGGAGATGGTGGATTCTCAGGAGAAAGAG
Seq A exon
GTGATAAAGGAATCCCAGGGATTCAAGGGGGAAGAGGAGATCCCGGGAGAGATGGACCGCCTGGGCTCCACAGAGGGCAGCCTGGGATGGATGGGCCTCCTGGGCCTCCTGGCCCCCCTGGCCCTCCAGGATCACCTGGACTGAGAGGAGTCATTGGTTTTCCGGGATTTCCAGGTGACCAG
Seq C2 exon
GGTGATCCAGGCTCTCCAGGACCCCCTGGATTCTCAGGAGATGATGGAGCAAGAGGACCGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014851-'48-55,'48-53,49-55=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
PF0139113=Collagen=PD(21.5=38.9),PF0139113=Collagen=PU(35.9=63.9)
A:
PF0139113=Collagen=PD(62.5=65.6),PF0139113=Collagen=PU(53.0=57.4)
C2:
PF0139113=Collagen=FE(31.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGGTGATGTAGGAGAAACAGC
R:
TTTCGGTCCTCTTGCTCCATC
Band lengths:
154-336
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]