BtaEX6011944 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021310 | COL4A4
Description
collagen, type IV, alpha 4 [Source:HGNC Symbol;Acc:HGNC:2206]
Coordinates
chr2:116018007-116020614:-
Coord C1 exon
chr2:116020507-116020614
Coord A exon
chr2:116018906-116019087
Coord C2 exon
chr2:116018007-116018070
Length
182 bp
Sequences
Splice sites
3' ss Seq
AACTCTTCCAACTCCATCAGGTG
3' ss Score
6.61
5' ss Seq
CAGGTAAGC
5' ss Score
9.88
Exon sequences
Seq C1 exon
GAGCAAGAGGACCCCCGGGATATGAAGGAGAAACGGATATTATTTCCAAGAAGGGGGAAACTGGAGAACCTGGACCTTCTGGGGATGGTGGATTCCCAGGAGAAAATG
Seq A exon
GTGATAAAGGTCATCCTGGGATGCAAGGAAGGAGAGGAGAGCCAGGAAGCCATGGACCACCTGGATTTCACCCTGGGGAGCCCGGAAGAAATGGACAGCCAGGGCTTCCTGGACCCCCAGGCCCTCCAGGCTCACCTGGGCTGAGAGGAAACATTGGTTTTCCAGGATTTCCGGGTGACCAG
Seq C2 exon
GGTGAGCCAGGTTCTCCAGGGTGCCCGGGACCTTCAGGAATGGATGGTACAAGAGGACCTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021310-'36-41,'36-40,37-41=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
PF0139113=Collagen=PD(20.7=32.4),PF0139113=Collagen=PU(35.6=56.8)
A:
PF0139113=Collagen=PD(62.7=60.7),PF0139113=Collagen=PU(54.4=60.7)
C2:
PF0139113=Collagen=FE(30.9=100),PF0139113=Collagen=PU(8.5=27.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCAAGAGGACCCCCGG
R:
TTAGGTCCTCTTGTACCATCCA
Band lengths:
170-352
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]