RnoINT0091766 @ rn6
Intron Retention
Gene
ENSRNOG00000006131 | Mettl2b
Description
methyltransferase like 2B [Source:RGD Symbol;Acc:1310240]
Coordinates
chr10:93354298-93356586:+
Coord C1 exon
chr10:93354298-93354389
Coord A exon
chr10:93354390-93356236
Coord C2 exon
chr10:93356237-93356586
Length
1847 bp
Sequences
Splice sites
5' ss Seq
AAGGTGCGC
5' ss Score
9.05
3' ss Seq
TCTTTCTCAAATGTTTACAGCTG
3' ss Score
7.92
Exon sequences
Seq C1 exon
GGACAACGTGAAGTGGTCAGAGGAGCAGGCCGCGGCGGCGGAGAGGAAAGTGCAGGAGAACAGTTCCCAGCTGGTGTGCCCGGAGAAGCAAG
Seq A exon
GTGCGCTGATTTGTCCACAGCCGGCAGTGCGGGACGCGCCTCCACGAGAAGGCAGTAGAAATGCGGGCACCAGGATCATTTGTTACTGACCTGATGAAGAACTCCGGCATAGAACGGGTCGGGGAGGGGACGAGCTAGGAGGCAAAGGGGCTGGCTGCCTTCTAACAAAATTTTGAAGGAAGTTGGGGTTGAGTTTTTGTATTCTCTAGTTCTACATTTTTAGTTCGTATTGACTAGTGTCATGTGAATGTAGCTGGCCCGTGGCAACCGTAAAGTCGTTCGATCATCTATCGTAGGGTAGCTTCGTCAGTCTGTGTTCTGCTGGAGAGTTTCCATTAGAGCTAGAATGATGTGGTCAGATCCTACCTACTGTTGGAGGAGAGGATGATTGGACCATGTATCTTTTCCTGGGGTGATGGGTTTGGAGATCTGGATCTTCTACCAAAAAAAGATCAGGCGCAGCAGAGTGGAGATGAGTCTGCTCAAAGAAGAAGAGGTCCTTCCTAAAGACAAGAATAATTTCTTTATAAAGTACTCTAAAGACTAAAACTAGAACTGGTTAGCCCTGTTTCTAAGAGACATTTGAATGTAAAACTAGAACTTTAAGATAATAGGCACCAGGGGACTGGGAGATGGCTAATAAGCATTGATAAAGTTTCTGCATAGTACTGAGGTCACAGTCCTGGCCAGGTATGGAACTCCTAGGATGGAGGGGGTGTAATCTCTTGGGGAGTGTGTCAAAGTCATCTTCGCTACATAGAGTTCCAATCCAGCCTTGGCTGCGTGAGAGCTTGTCTCAAAAAATATATATAAGCATGTGTATATATTCCAGTTGCGAAGTAGGCCTCCTACCTGCATTGTTAAATGCAGACCAGATAAAGCATCATAGTGTTCAGAGGGATTTTCAAAGTCATGTAGCCCAATATCCTCTTTCTACAGTTAAAGAACCAGTTAGCCAAGTGCAGTGGTGTATAGACTCAGCTATGTGGGAGGCTGAGACAGAAGGACCACTGAGTTGGAGGCTGTCCTGGGCAGCATAGGGTGATGTAGTCTCCAAAAAAATTAAGCTTGAACCACAGGGCCTTAAGCGCTTTGCCTGGAGGTAAGCAATCAGTGGTGGGCTCCCATTGCTTGGGTCTTCATTCTTCCATCAGGTCTGGTCCAGGTCTTCGTGTCAGCAAACGGAGAAAGAGCCCCTATCAGTCTCCTGATTAGCCGAAACTCTTCTTGCTTCGGGAAGAAGAGTTTTCTCACTGTCTGGTTTTCCTCATATTCTAGTTATTTATCTTAGCATTCATTACTAATCTTGACTTTGTGCCTGGCCCCCACGTTCTAAAGAAGCAGGAACGTGCCTTCCGAAGAGATGCATGAGTGACAGTCACTGGATTTACACAGATATCCAAAGAGCCTGAGACATTTAACTTGTAAATTAAAAAAAAAACTTACAAGGGAAAATAATTACCACAGACACCAACGAACCTGCCTGTCCCTGGCAAATAGAACCTTGAAACTAAGAATAATTAGACTTGTGTGCAGTGCTTGGGGTTTGCTTAATTTCATAATTCCCCCGTAGTCGAAGGCCATCCAGAGCTGTGTCAGGAAAATGATGGGTAGCTAACTCCCTGTCTGATGGCAGAGGCTAGGCTCCCTGGGGATGAAAAGTGGGTGACGTAAACGTAAGATGGATAGAAGGCGATTGCCTGTGAAGGTGCCCAGTTCTCAGACCCTGACTGCCCTCAAGCATACTTCTGGAATAAAATGATGTTTGGTGATGCAAGACACTTGACATTAGATATAAATAAAATATTTGAGACCGACTCTTAAAATTCTTTCTCAAATGTTTACAG
Seq C2 exon
CTGATTATGAAGTCAATGCCCATAAATACTGGGATGACTTCTACAAAGTTCACGAAAATGGGTTTTTCAAGGACAGACACTGGCTTTTTACTGAGTTCCCTGAACTGGCACCTAGCCACGATCACCTAGTGAATTTGCACTTGGAGAAACAGAAGAATGAAGTCTCCAAACCTAGAAGCAGTGAGGATGGACCTGGCTTGGCAGCAGAACAACACAAGCGTCCTTGTACCAGCCATGGATGTGAAACTCGGGTGCCTCCCGTGGAAGAGCCGGTGACTCAGAAGCTCACCCACCTGGAAATTTGTGCTGACGACTTTCCTGGCTCCTCTGCTACCTACCGAATACTTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006131:ENSRNOT00000008140:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.750 A=NA C2=0.282
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF134891=Methyltransf_23=PU(15.4=24.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGCAGGAGAACAGTTCCCAG
R:
TTTCCAGGTGGGTGAGCTTCT
Band lengths:
343-2190
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]