RnoINT0097208 @ rn6
Intron Retention
Gene
ENSRNOG00000030022 | Myo3b
Description
myosin IIIB [Source:RGD Symbol;Acc:1560313]
Coordinates
chr3:56583059-56584547:+
Coord C1 exon
chr3:56583059-56583221
Coord A exon
chr3:56583222-56584467
Coord C2 exon
chr3:56584468-56584547
Length
1246 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
3' ss Seq
ACATGGTTTTATATTTTTAGCCT
3' ss Score
6.06
Exon sequences
Seq C1 exon
GTTTTTCTCAAATATTACCACATCGAGCAGTTGAATTTGCTTCTTCGGGAAGTTATGGGCCGAGTGGTCATGCTACAGGCATATACCAAGGGTTGGCTCGGAGCGAGGAGATACAAGAGAGCCAAAGAGAAGAGAGAGAAGAGCGCCATAACCATCCAGTCAG
Seq A exon
GTAAGTGGCCTGGTCTGGCCAGTGCCACCCCTCACCATTTGAGTGCTGGGATCACTCACCTTGGGCTTCCCCTTTTGCTGTCTCCCTTCCTCCCCTTACTTCATCAAACATTTTCCAAACTCCTATACAAGTCAAGCCTCATACTAGGCACTGGGAATGTGGTCAAAGGAAACTCTCTGATCTTAAAGACAGACGGACATTCAACCATTATTACACCCTGGCGCGAGTGGTGAGACAGCAGTATGAACTGACCAGTGGTGTAAGACCCCATCACCCCACGTGTGCCTGTCTCCTGCAGTGGCTGAGGTAATGCACACTTCGAGTGCTATAGAGATTGTCCCATCACAATGGCTAGCACCATCCTAGTAGGGTTTGGTAGCACAGCTGAGACTGAGAAACAGGAAACAGGCCTCTAGGCTTTACCCGAGCCCACTGACACCTCTGTTCATGTGCATATCATGGGTGCTGAGAACAGTACCCGACTCTCATGCTCCTGAGGATGAGACAGGCATGGAATGCAGACGCTGAATTTCCTGGCACTCCCATATCAAGAAACCATAGCATGTGTGCTTTGGACCTCAGGAGCCGTTTCTGAGATGCTCAGGCCAACATGAGGACACAGAAGACAGCCTCCCAGCTTAGGGTGGAGGCTTCGTGCATCACCTTTATCTGAAAGATGTCAGTCTCCCGCACTCTGTTCTCTTGGCTCTTGGCTTACTTCCTTACCTTCCTATTCTATGGGAAATGTGGCCACATCCTCAAAAAGCCCTATGTGCTGAGCGCCTTCCTCTGCTCCTTTATCTTCATCCCCAATTATACGTTTCCTCTGAACCTAAAAGGAATGAAAGGACTAGTTCAGAGCCTCTCGCCATCCTCCCAAGCCCTGACCACCAGTTTTAACCCCTGTGCCCAAGACAGGGCTTTCTCCATTCTTGAGATAACAGGAAACCACCTCACCTGGAACCAGGGCCTCTCACTATTACTAAACAGAACTCAAAATGTTCTGGGAAAGTTTGCCTAAAAATGGTTCCTCCGAAGCATTTTGAAAACTTGACCAAGAGTTCAGAAAGGCCCTTCCTCCACTGCAGTTCATCTGCTGACTTTGATTTTCCTCGGGGAGAAGGCAGGGTGGGGGTGGGGGGCTTGGGGGATGAGAGACGAGGATTGGAGGTAGAACAGCTTCTATCAATTCACTGGCACGTTTGTAAATTAATGTCATTCCCAGCACATGGTTTTATATTTTTAG
Seq C2 exon
CCTGGAGAGGATATGATACTCGGAGGAAATTTAAGCAAACAAGGCACAGGAGGAACGAGTCTGCAGCTCACACTCAAGCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000030022:ENSRNOT00000037188:26
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.556
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=5.5),PF0061222=IQ=PU(42.9=16.4)
A:
NA
C2:
PF0061222=IQ=PD(52.4=40.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCAAATATTACCACATCGAGCAGT
R:
TGCTTGAGTGTGAGCTGCAG
Band lengths:
238-1484
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]