BtaINT0098139 @ bosTau6
Intron Retention
Gene
ENSBTAG00000003626 | MYO3B
Description
myosin IIIB [Source:HGNC Symbol;Acc:HGNC:15576]
Coordinates
chr2:25973378-25974912:-
Coord C1 exon
chr2:25974750-25974912
Coord A exon
chr2:25973467-25974749
Coord C2 exon
chr2:25973378-25973466
Length
1283 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAC
5' ss Score
7.82
3' ss Seq
ACATGGTTTTATATTTTTAGCCT
3' ss Score
6.06
Exon sequences
Seq C1 exon
GTTTTTCTCAAGTATTATCATGTTGAGCAGTTAAATTTGCTGCTGCGGGAAGTCATAGGCAGAGTGGTTGTGCTGCAGGCCTATGCCAAGGGGTGGCTGGGAGCCAGGAGATACAGAAGAGTCAGAGAGAAGAGAGAGAAGGGGGCTGTTGCCATCCAGTCAG
Seq A exon
GTAAACATCCCTGAATCTTGGCATTTCATTAAGTGCTTGAGGCCACTCACCTTTTGTTCTTCTCAGTGGTGCCTTTTCTTCCCTCCCTCTTGCCTTCAGCAAATATTTATCAAGTATCTACTGCAGGCCAGGCCTTGTGCTTGGTGCTGGGGGTATAGCTGTGAGCAAGACTGCCACAGTCTCTGACCTCCAGATAAGCAAACGAGCCATCCTTACCCACGGCAAGATAGAGGCAGTAGCAGTGGGTGGATGTAAAACCCCAATCACACAGACATGTGCCCCAGCTCCCAGATGACCCACCTCATGCACACTTCATGTCCATTACCAGGTTTTCAGCCTCAGAACTGCTACTGTCACATCTAGGAGGCTTTCCTGGCTCAGACAGAGCTTGTAAACCAAGGCAGCTTATCACTAGGCTCTGTCCTACACCCAGCCCTGTCAGTGTCTCTTTGCACACACATGTGTCATTTGTGTCACAAACAGCGTCTGAGCTCCTGTGCTACTGCAGGCAGTTGTCCCAGGTCTTGAGCACAGCAGATGATTTCCTTTGTGTTCTCATAACCCAGAATCATAACGTCCACATGGACTCCATGACCTCATTCAGTCCCATGGAGAGCTGTAGCGCTCAGGGAGGAAGTGAAAAGGAGAGCTGGTCATGCTGCTGGTGGCAGCTCAGTGCCTAGCGCTCTGAAGGACTCAACACCCTGCCCCTTGCCCCAGGCTCCCTTTCTCTTCTCTTCATTCATTTCCCACGCATTTTCCCTTTCCGTCACGCATTCCCTCCCTGAACACAGTTCTTTACATAGTCTCCCCTCACCAAACGCAAATGTAATGCTACTAATCATTATTATTTATTTTTCTCTCCTTCAACTTTCATGTTTTATACCTTTCTCCCAATAAAAAATACAGAGTGAGTCAGGCCATTCCAGTCCTCTCCTACCCCTCAAGTCCTGAAGGCAGAGCTTCCTCCATGGGTTGGAATGACAGGGACCCACCTCACCTGCACAGGGCTCCAAGTGATTCCTAAGTGGAATCCAGAGGTTGCAGAAATTCACTGCCTGAACCAAAATGACTCCTTCTCAAAAGCATCTTGGAGGCCGAGCTGAGAGTACAGAGAGGCCCCTCTTCCCCACCATCACTCACGTAGCTCTGCTTATTTTGTTCAGTGAGAAAGCAGTGGGGAGGAGGAGAGGGGTAGAGGAGTGGAGGGAAACAGCTTATATCAATTCTCTGGCACATTCTTAAATTAATCTCATTCCTAGCACATGGTTTTATATTTTTAG
Seq C2 exon
CCTGGAGAGGATATGATGCTCGGAGGAAATTTAAGAAAATAAGCAACAGAAGGAGTGAGCCTGCTGTTCATATTCACGCAGGTAATTAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000003626:ENSBTAT00000061222:26
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.069
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=5.5),PF0061222=IQ=PU(42.9=16.4)
A:
NA
C2:
PF0061222=IQ=PD(52.4=36.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTCTCAAGTATTATCATGTTGAGCA
R:
ATTACCTGCGTGAATATGAACAGCA
Band lengths:
246-1529
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]