RnoINT0097216 @ rn6
Intron Retention
Gene
ENSRNOG00000058866 | Myo5a
Description
myosin VA [Source:RGD Symbol;Acc:3143]
Coordinates
chr8:82070462-82071920:+
Coord C1 exon
chr8:82070462-82070727
Coord A exon
chr8:82070728-82071838
Coord C2 exon
chr8:82071839-82071920
Length
1111 bp
Sequences
Splice sites
5' ss Seq
TGGGTAAGT
5' ss Score
10.24
3' ss Seq
TTACTTTTTCGGCATCACAGATT
3' ss Score
6.45
Exon sequences
Seq C1 exon
CCCAAGCACGAGCCTCTTATCATTTTCTGTGATCTTATGGGTGTGGATTATGAAGAGATGTGTCACTGGCTCTGCCACCGAAAGCTAGCTACCGCCACAGAGACGTACATCAAGCCCATCTCCAAGCTGCAGGCCACAAATGCCCGAGACGCTTTAGCAAAGCATATCTATGCAAAGCTCTTTAACTGGATTGTCGGCCATGTCAATCAGGCTCTCCATTCAGCTGTCAAGCAGCACTCTTTCATCGGCGTGCTGGACATTTATGG
Seq A exon
GTAAGTGCGTTTGGCCGGCTGGCCTACCCTGTTGGAAAGCAGTTGTTTATTTGGGGGTGTTTGCTTGAGTACTGTTTAATTCTGTTCTCTATTCCACTATCTTTTTTTTATTGGATATATTTCTTATTTACATTTTAAATGTTATTCCCTTTCCCAATTTCCTGTCCATAAGCCCCCTAGTCCCTCCCCCTCCCCCATAAGGGTGTTCCCCCACCTCCACCCCCCCTTACCACCACCACCCTGACATTCCCCTGCACTGGGGATCCAACCTTGGCAGGACCAAGGGCTTCCCATTCCACTGGTGCCCCAACAAGGCTATTCTCTGCTACATATGCAGTTGGAGCCCTAGGTCAGTCCACGTACAGTCTTTGGGTAGTGGTTTAGTCCCTAAAAGCTCTGGTTGGTTGGCATTGTTGTTCTTACGGGGTTGCAGGCCCCTTCAGCTCTTTCAATCCTTCCTCTAATTCCCCCAAATGGGGTCCGGTTCTTAGTTCAGTGGTTTGCTGCTTCTATTCCAGTATCTTGATGGAGGTATAATGATACAAAAAAAATCACACATTTGCTTAAACAAAAGTTAACTCAGCTCCAGAGTCCTTCTACTAAGCTAATAGGTAGACTTAATTGGTTTTCAGACTCTCATTTGATAAGTTACTGAATTTCTAAAAATATAATTTCATTTCTAGTTGCCTTATCTGAATACACTTCTGTAAATGTTGAAACTTGTTTGGCAGCTAATTTTCACTCTCTGGTTTAGTTTTTCAGAAGAACTTATTAGGCTTCTGTTTCTACCTCCCGCTTCTGTGTGAAATGTGGAGTATAAATGGACAGGAACTGTGGCTTTAGCATAGTTCTAGTGTTTCCTGCTGGAGGATAAATGAGCCTCAGAATCCCACGGCTGCTGGTCCTTAGGCAGGGTTGTACAAAGGGCAGCTTCGCCTCAGCGGAGCTCATCCTGTTCTGTCTTTCCAGCTTAGACCTTTAACTGAATGTAAAGGAAAATGTAAAAATGTTACGAGACTTCCCATTTTTCTCTTCCAAGTGTGCATATTTTAAATATTTTTTAAAACTATTTTTTTTCTTTTCTTCCTACCTTACTTTTTCGGCATCACAG
Seq C2 exon
ATTTGAAACATTTGAGATAAATAGTTTTGAACAGTTCTGCATAAATTATGCAAATGAAAAGCTACAGCAACAATTCAACATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000058866:ENSRNOT00000079535:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(12.9=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(4.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCAAGCACGAGCCTCTTATC
R:
CATGTTGAATTGTTGCTGTAGCT
Band lengths:
348-1459
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]