RnoINT0097237 @ rn6
Intron Retention
Gene
ENSRNOG00000058866 | Myo5a
Description
myosin VA [Source:RGD Symbol;Acc:3143]
Coordinates
chr8:82043402-82045524:+
Coord C1 exon
chr8:82043402-82043573
Coord A exon
chr8:82043574-82045379
Coord C2 exon
chr8:82045380-82045524
Length
1806 bp
Sequences
Splice sites
5' ss Seq
GTGGTGAGC
5' ss Score
6.64
3' ss Seq
CTCTTGCCCTCCTCCCCCAGGGA
3' ss Score
11.4
Exon sequences
Seq C1 exon
GATTTGGAATACCGTCTAGATCCAAAGACCAGCGAGCTCCCTCACCTACGGAACCCTGACATACTTGTTGGAGAAAATGACCTCACAGCCCTCAGCTACCTTCACGAGCCTGCTGTGCTGCACAATCTCCGCGTCCGCTTCATCGACTCCAAACTCATTTATACGTATTGTG
Seq A exon
GTGAGCTTTGCCGTTGAGCTGTAAGCTTTCCTTTGATGGCTGTAGTCACTGAAATGCACACAGGGAAAGCCAACTGTCACTGAATTTCTCAAACATGCAAAGATCCCTTCTAAGTTATCAGAAAGATTTATCAGTATTCACTAGCTGGCTACATTTCTTGTCAAACACAGTCATTTGTGAATATACATAATTTTGCTCAACATTTAAAGAACAGGTCCCTCTTTGGGATTCTCCCTTCCAAGTCATTTCAGGTTTAGTGCTGAAGTTGCTAGTTCTCTTTTTTTTGTGAAAGTCTGCTCTATGAAGTGTAGATGTTGAAGGACTGGGATTTGGTGCAGGTGCAAGGTTAGTGCTACCTTTCTTCTCAGTAACTTGGACACCCAGGACTGACACCTCCTGGTGTCTGTCTCCCTCCTGCAGTGTGAGCTGCCAGCAGCCCCATTCCTGGAGCCTGGGCGTGTGGCTGTGGACCTTCTCCCAGCCCCATCTGTCTTTGTTTTTCCCTGTTCTATTTTGTTCTTACTGATTTCCTCTCAGCTCTCTGGCTCCTTTTCTCTCATGATCCTCTCTCTCCTACTAATGGCGACTCTGCTGAGGTCCAGACGGTCATTCACTCCCATGTGTTTGTCGGCTTGCTGACCCGTGTTGTGTGTCATGTCTTCACTCTTGTCTGTTTAAACAGATACAGACACTTTCTCCTCTCAGAAGAATTTATATCTAATGTATCTGTCTTGATAACATTTCTTTTCCTTATTATTTAAATATTTTTCTTATTTTCTTCAACTTATACTTTTAAATGTATTTTATGTGTGTAAGTATTTGGCTGCATGTATGTGTGTACACCACACGTGTAGTGTGCCAGGTGCCTGTAGAGGTCAGAAGAGAACTCTGGAGTTTCAGATGGTTATGAATCACTGTGTGATGCTGGGGACCAAACCTGGTCCTCTGCTAGAGCAGTGCTCTTAACCGTTGAGCCATTTTTTCATACCCTCAACCTATTTATTAATTTGTACATGCCATGTTTTTCTGTGATGTGTTTTTTCTTTTGAGACCAGTGTCTCCTGTAACCCAGACTGTCTGAAACTCATTGTGTAGCTGAGAATGACCTTGAGCTTCTGATCCTCCATCTGTCTCTACTCTGACATGTAATATTCTTCAAAAGCATGTATTTTGTAATGTGGCATAACGGCTTAAGTAGAATCACAAAGATTTTAGTATGTATCTTGTTTGTTTCTGTTTGCTGTGTGACTCCCAGTGTGATAGCTTTCTAAAAGAAAATCCTTCAGATGAATCTCTGATTATTTCATTGGATAAATTTTTATAAATGAGCTTTGGAATTTTTTATTTTTATTGGGGAGGTGTATTCCATGCCTGTGGGTACTCACAAACACTAGAAGATGGTCTGGTTCCTCAGAGCTGTAGTTCCAGGCAGTTGTGAGTGGCCTGACATGGGTGCTGGGAACTGAACTCACATTCTCTGCGAGATCAGCAAGCGCTCCTACCCCCGAGCCTTCTCTTCAACCCCCCTTTTTTACTAAGTATGAAAATATTTAAATTATTTAAAACTGTTTCTTTTTTGCCACATGGAGATCAAACCCAGAGCTTCACAGATGATTGACAGGTGTTCTGCTTCTGAGCTCCACCCTGTCTAAGTGACATTATTTTAAACCATCTGGTCCAGATGATTTAGACTACCTAAGACTAGTATATTTTGATCTTTGAGCAGTTGCTGCCTTACTTTACCAAGCCTAATGTGCCCATTAGGAGATCACTGATTGCATTTTCTCTCTTGCCCTCCTCCCCCAG
Seq C2 exon
GGATAGTCCTGGTAGCTATAAATCCCTATGAGCAGCTGCCTATCTATGGAGAAGATATCATTAATGCATACAGTGGCCAGAACATGGGTGACATGGACCCTCACATCTTTGCAGTAGCTGAAGAGGCCTATAAACAAATGGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000058866:ENSRNOT00000079535:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.017 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PU(24.3=56.9)
A:
NA
C2:
PF0006316=Myosin_head=FE(35.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAATACCGTCTAGATCCAAAGACC
R:
TGCCATTTGTTTATAGGCCTCTTCA
Band lengths:
310-2116
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]