RnoINT0099854 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37678278-37679096:-
Coord C1 exon
chr3:37678992-37679096
Coord A exon
chr3:37678371-37678991
Coord C2 exon
chr3:37678278-37678370
Length
621 bp
Sequences
Splice sites
5' ss Seq
TCGGTATAT
5' ss Score
4.42
3' ss Seq
TCCTGATTCTGAATGCCCAGGTT
3' ss Score
3.47
Exon sequences
Seq C1 exon
CTTCAATACCAGTGTGACCTTAAAAACAGTAAGGGGAAAATTACAGTGGTCCAAGACACGCCAGAAATACTGCGTGTTAAAGAAAACCAAAAGAATTTCAGCTCG
Seq A exon
GTATATTTACTGTTTCCTTTCCTTCTAATGCTAACCTAATAATGCCGTTCTCTTCAAGAAGGGACACGGGCCCATGCTGACCCAGGCAATTAACCTCTAACTTGCCCAGTTAATGCTAATGGGTCTCTGATAAAAGCCCCCAAATTTCTAAATCATTACTCCTGAGAATATTTGGAATAACTTAACTCTAACCAAATAACTCTTCTCTTTGTATTTTTAATCTCAAAAAGTAACTCAGACAGTAACACACATTGTCTTGTTCCAAGAAGGTGGATTTCTTTGTGATGTGGTTTTCTTTTAATAGCCTTCTCCAAAATAACTGACTGACCATGCGTTTCTTTAATGAGTTCTGTGGTTTTTTATCGTGTTTTTTTCTCATCCTGTGTTAATATCCTGTGATATTCTAAATAGAACTCTCAAGAGCTGGAAACTGGCCGGCCTGGCCTATGTGGTTTCAGGCTTTGGTTCTGACAGGAGGACATCAGATGCTGTCCTTCAGCTCTTGTCTGGAAACACCGTGTAGTGTCTTGGTTTTCACAGAGGCCTGGCACTGCCCTTTCTCCAGGCACTGCCCAGGTTCCCCTCTTTGGGGTTAACACTGTCCTGATTCTGAATGCCCAG
Seq C2 exon
GTTTTATATAAAGAGGATGTGTCCCCGGGAACAGCAATCGGAAAGACTCCGGAGATGCTCAGGGTGAAGCAAACTCAGGACCACATTAGCTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:100
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.057 A=NA C2=0.581
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(66.7=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(25.0=19.4),PF0088013=Nebulin=PU(61.5=51.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTCAATACCAGTGTGACCTTAAAA
R:
CGAGCTAATGTGGTCCTGAGT
Band lengths:
198-819
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]