MmuINT0107212 @ mm9
Intron Retention
Gene
ENSMUSG00000026950 | Neb
Description
nebulin [Source:MGI Symbol;Acc:MGI:97292]
Coordinates
chr2:52016176-52016995:-
Coord C1 exon
chr2:52016891-52016995
Coord A exon
chr2:52016269-52016890
Coord C2 exon
chr2:52016176-52016268
Length
622 bp
Sequences
Splice sites
5' ss Seq
TCGGTACAT
5' ss Score
4.33
3' ss Seq
TCCTGATTCTGAATGCCCAGGTT
3' ss Score
3.47
Exon sequences
Seq C1 exon
CTTCAATACCAGTGTGACCTTAAAAACAGTAAAGGGAAAATTACAGTTGTTCAAGACACGCCAGAAATACTGCGTGTTAAAGAAAACCAAAAGAATTTCAGCTCG
Seq A exon
GTACATTTACTGTTTGCTTTCCATCTAATGCTAACCTAACAATGGCGTTCTCTTTAAGAAGGTCCGTGGGCCCATGCTGACCCAGGCAATTAACCTCTAACTTGTCCAGTTAATGCTAGTAGGTCCCCGATAAAAGCCCCCAACTTCTAAATCATCACTCCTGAGAACATTTGGAATAACTTAAATCTAACAAAATAACTTTCTTTGTATTCTTTAATCTCAAACAGTAACTCCGACAGTAACACACATTGTCTTGTTCCAAGAAGGTGGATTTCTTTGTGATGTGGTTTTCTATTAGCCTTCTCCAAAATAACGGACTGACTGTGCATTTCTTTAATGAGTTCTGTGGTTTTTAATCGTTTTGGGTTTTTTTTTCTCATCCTGTATTAATATCCTGTGATATTCTAAATAGAACTATCAAGAGCTGGAAAGTGACCAGCCTTGCCTATATGATTTCAGGCTTTGATTCTGACAGGAGAACATCAGATGCTGTACTTCAGATCTTGCCTGGAGATACCGTGTATTGTCTTGGTTTTCACAGAGGCCTGGCACTGCCCTTTCTCCAGGCATTGCCCAGGTTTCCCTCTTTGTGGTTAACACTCTCCTGATTCTGAATGCCCAG
Seq C2 exon
GTTTTATATAAAGAGGATGTATCCCCGGGGACGGCAATTGGAAAGACGCCTGAGATGCTCAGAGTGAAGCAAACGCAGGACCACATTAGCTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026950-Neb:NM_010889:143
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.114 A=NA C2=0.564
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(66.7=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(25.0=19.4),PF0088013=Nebulin=PU(61.5=51.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTCAATACCAGTGTGACCTTAAAA
R:
CGAGCTAATGTGGTCCTGCG
Band lengths:
198-820
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: