RnoINT0118696 @ rn6
Intron Retention
Gene
ENSRNOG00000005336 | Prss27
Description
serine protease 27 [Source:RGD Symbol;Acc:1303256]
Coordinates
chr10:13415183-13416860:+
Coord C1 exon
chr10:13415183-13415345
Coord A exon
chr10:13415346-13416588
Coord C2 exon
chr10:13416589-13416860
Length
1243 bp
Sequences
Splice sites
5' ss Seq
CAAGTAAGT
5' ss Score
10.08
3' ss Seq
AGCTCTGCTCTTTCCACTAGCAC
3' ss Score
7.1
Exon sequences
Seq C1 exon
CCTGTGGGCATCCAAGGATGTTTAACCGGATGGTGGGCGGGGAGGATGCCTTAGAAGGTGAGTGGCCCTGGCAGGTCAGCATCCAGCGCAATGGAGCTCACTTCTGTGGGGGCAGTCTCATCGCACCAACATGGGTCCTCACTGCTGCTCACTGCTTCTCCAA
Seq A exon
GTAAGTAGCCCTGACCCTTTCAGCTTCTCACCAGTGGTTGGGACAAGACAGAGCCTGTGCGCACAACTGTTCAGCACCACGGACAGCACTTCCAGCTGCACACCCCACCCCCACCCTATGCCTGGTCCAGTAACACCCTCCATCTTCCAAAGTTTTCTTTTACGGAAAGGGACAGACAGCCCAGTGTACTGGGTGTTCTTGTAGAGAACCGATGTTCAACTCTCCACATCCCCATGGTGGCTCACAACTTTCTATAGCTCGATTCCAGATTAACACCCTCTTCTGACTTAGGCAGGCTTCAAGCATGCGCACGGTGCATGGACACAAAACATTCATACACATAAATAATTTAAAGTTTTCCTTTGGCTTCGACCCTATGCCTGGAAGCTGCAACTACTGCCCCAGTTCCTGCTCCACCCGCTGCTGTCAGTGATATCTGGGCTCCTGTTCTTGCAGGCACAGCCCCACCTCCCTTCACACAGAATGAGAGCTCTCTCTGGCTTGGGGATATCAGATAAGCCTAAGATTTGGAACAGAATGTCCCTCTTCCTCACCCCATTTAAGACCACCTCAGAGGATAGTTCCCAATCCACACATCCATGTGAGGTTCTGGCCTTGGATTTTCAGGGTCTCCAAACATTATACCTCTTTCCTCACAGATCTGAAATTCCTCTCTTCCTTTTATTTATTATTGGGTGTTTGGGGGTGGGGCACGTGTCCCGGTGTGGAGGTCAGAAGACAACAATTCAGAGTCGGTTTTCTCCTGTCACTACGTTTTTGTGTGGGCGTGATAAAACCTGGGCATTTAGGCTTGCACAGAAAACACCTGTACCTACTGAACCATCTTGCCAGCCCCTGCAATTATTTTAAAAAGCTCAAAAGTGGCCTACCTGGAGCTCAAAGGCAGGCCTTGGGCTCACTGTACCTCAGAGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTTACAGTAGGCTTTTCAGGTAGGATTCTTAGTGCTCCTGTCTTACAAATGAGGAAACTGAGTCACAGGGAACTTGTTAGGTCACCTGGCCACCTGGTAGCCAGGAAAAGATGTTGCAGGGGGCAGGGGGAAGATTTTCAGATTCTACAGTCTTTATCAGGGAGATGACATTTTACTCATGCAAAGAGAAGTGTCACATACTGTGGGTCCCTGTCCTCGTGGAGGAGAGTACTCACTCACACCAGCTCTGCTCTTTCCACTAG
Seq C2 exon
CACATCAGACATATCCATATACCAGGTCCTGCTGGGGGCACTGAAGCTGCAACAGCCAGGACCACATGCCCTGTATGTCCCCGTGAAGCGGGTGAAAAGCCATCCTGAGTACCAAGGCATGGCTTCCAGTGCCGACGTGGCTCTGGTGGAGCTGCAGGTGCCTGTGACCTTCACCAAATACATCCTTCCCGTGTGCCTGCCTGACCCTTCGGTCGTTTTCAAGTCGGGCATGAACTGCTGGGTCACCGGCTGGGGCAGTCCCAGTGAGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000005336:ENSRNOT00000007392:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=PU(18.5=80.0)
A:
NA
C2:
PF0008921=Trypsin=FE(38.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Mouse
(mm10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTTAGAAGGTGAGTGGCCCT
R:
CCCGACTTGAAAACGACCGAA
Band lengths:
343-1586
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]