BtaEX0021380 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000010271 | MAPKAP1
Description
Bos taurus mitogen-activated protein kinase associated protein 1 (MAPKAP1), mRNA. [Source:RefSeq mRNA;Acc:NM_001081603]
Coordinates
chr11:96323245-96372659:-
Coord C1 exon
chr11:96372550-96372659
Coord A exon
chr11:96338889-96338996
Coord C2 exon
chr11:96323245-96323385
Length
108 bp
Sequences
Splice sites
3' ss Seq
CTGCCCCTTTGATCTTCCAGGCC
3' ss Score
11.02
5' ss Seq
ACAGTACGT
5' ss Score
7.54
Exon sequences
Seq C1 exon
AAATGCTGCTCATGGATTCTCCCTTATTCAGGTGGACAACACAAAGGTCACCATGAAAGAAATCTTGCTAAAGGCAGTGAAGCGAAGAAAAGGATCCCAGAAAATTTCAG
Seq A exon
GCCCTCAGTACCGCCTGGAGAAGCAGAGCGAGCCCAACGTCGCCGTGGACCTGGAGAGCACCTTGGAGAGCCAGAGCGCCTGGGAGTTCTGCCTGGTCCGCGAGAACA
Seq C2 exon
GTTCAAGGGCAGACGGGGTTTTTGAGGAGGATTCGCAGATTGACATAGCTACAGTACAGGATATGTTGAGCAGCCACCACTACAAGTCATTTAAAGTCAGCATGATCCACAGACTCCGCTTCACAACCGACGTCCAGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000010271_MULTIEX1-9/9=8-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.053 A=0.243 C2=0.000
Domain overlap (PFAM):
C1:
PF054227=SIN1=FE(8.0=100)
A:
PF054227=SIN1=FE(7.8=100)
C2:
PF054227=SIN1=FE(10.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTGCTCATGGATTCTCCCT
R:
AACTGGACGTCGGTTGTGAAG
Band lengths:
246-354
Functional annotations
There are 1 annotated functions for this event
PMID: 16919458
[No functional differences found]. Immunoprecipitation demonstrates that mSin1.1 (all included), mSin1.2 (skipping of HsaEX0037808), and mSin1.5 (AI and APA) integrate into mTORC2 complexes. Insulin treatment increases the kinase activity of mSin1.1 and mSin1.2 but not mSin1.5 complexes.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development