GgaEX0000990 @ galGal3
Exon Skipping
Gene
ENSGALG00000000977 | SIN1_CHICK
Description
NA
Coordinates
chr17:10379121-10401440:-
Coord C1 exon
chr17:10401331-10401440
Coord A exon
chr17:10385703-10385810
Coord C2 exon
chr17:10379121-10379261
Length
108 bp
Sequences
Splice sites
3' ss Seq
CAAGTTACTTTATGTTTCAGGTC
3' ss Score
9.15
5' ss Seq
ACAGTACGT
5' ss Score
7.54
Exon sequences
Seq C1 exon
AAACGCTGCTCATGGATTCTCACTTATTCAGGTGGACAGTATGAAGGTCACCATGAAAGATATCTTGCAAAAAGCCTTAAAGAGAAGGAAAGGATCACAGAGAGGCTCAG
Seq A exon
GTCCTCAGTATCGGCTGGAGAAGCAGAGTGAACCTAATGTCCCAGTAGATTTAGACTGTACCTTGGAGAGCCAGAGCACTTTGGAATTCTGCCTTGTCCGAGAGAACA
Seq C2 exon
GTTCGAGAGGAGAGGAGATCTCAGAGGAGGACCCTCAGATCGACATCGCCACGGTGCAGGACATGCTCAGCAGCCACCACTACAAGTCCTTCAAAGTCAGCATGATCCATAGGCTTCGATTCACCACTGATGTTCAGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000000977_CASSETTE2
Average complexity
S
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.193 A=0.351 C2=0.174
Domain overlap (PFAM):
C1:
PF054227=SIN1=FE(8.0=100)
A:
PF054227=SIN1=FE(7.8=100)
C2:
PF054227=SIN1=FE(10.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACGCTGCTCATGGATTCTCA
R:
ACATCAGTGGTGAATCGAAGCC
Band lengths:
242-350
Functional annotations
There are 1 annotated functions for this event
PMID: 16919458
[No functional differences found]. Immunoprecipitation demonstrates that mSin1.1 (all included), mSin1.2 (skipping of HsaEX0037808), and mSin1.5 (AI and APA) integrate into mTORC2 complexes. Insulin treatment increases the kinase activity of mSin1.1 and mSin1.2 but not mSin1.5 complexes.