MmuEX0027866 @ mm10
Exon Skipping
Gene
ENSMUSG00000038696 | Mapkap1
Description
mitogen-activated protein kinase associated protein 1 [Source:MGI Symbol;Acc:MGI:2444554]
Coordinates
chr2:34533788-34581332:+
Coord C1 exon
chr2:34533788-34533897
Coord A exon
chr2:34563055-34563162
Coord C2 exon
chr2:34581192-34581332
Length
108 bp
Sequences
Splice sites
3' ss Seq
CTGCCCCTTTGATCTTCCAGGCC
3' ss Score
11.02
5' ss Seq
ACAGTACGT
5' ss Score
7.54
Exon sequences
Seq C1 exon
AAATGCTGCTCATGGATTCTCCCTTATCCAAGTAGACAACACAAAGGTCACCATGAAGGAGATCTTGCTCAAGGCAGTGAAAAGAAGAAAAGGATCCCAGAAAATTTCAG
Seq A exon
GCCCTCAGTACCGCCTGGAGAAGCAGAGTGAGCCTAACATCGCTGTGGACCTGGAGAGCACGCTGGAGAGCCAGAACGCCTGGGAGTTCTGCCTGGTTCGCGAGAACA
Seq C2 exon
GTTCAAGGGCAGACGGAGTTTTCGAGGAGGATTCACAAATTGACATCGCTACAGTACAGGATATGCTTAGCAGCCACCATTACAAGTCATTCAAAGTCAGCATGATCCACAGACTGCGATTCACGACGGATGTGCAGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000038696_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.048 A=0.162 C2=0.010
Domain overlap (PFAM):
C1:
PF054227=SIN1=FE(12.1=100),PF054227=SIN1=PU(1.6=5.3)
A:
PF054227=SIN1=FE(12.5=100)
C2:
PF054227=SIN1=PD(1.3=8.3),PF054227=SIN1=FE(36.7=100)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTGCTCATGGATTCTCCCT
R:
ACTGCACATCCGTCGTGAATC
Band lengths:
245-353
Functional annotations
There are 1 annotated functions for this event
PMID: 16919458
[No functional differences found]. Immunoprecipitation demonstrates that mSin1.1 (all included), mSin1.2 (skipping of HsaEX0037808), and mSin1.5 (AI and APA) integrate into mTORC2 complexes. Insulin treatment increases the kinase activity of mSin1.1 and mSin1.2 but not mSin1.5 complexes.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types