RnoEX0052619 @ rn6
Exon Skipping
Gene
ENSRNOG00000017583 | Mapkap1
Description
mitogen-activated protein kinase associated protein 1 [Source:RGD Symbol;Acc:1305363]
Coordinates
chr3:13602245-13662380:+
Coord C1 exon
chr3:13602245-13602354
Coord A exon
chr3:13645148-13645255
Coord C2 exon
chr3:13662240-13662380
Length
108 bp
Sequences
Splice sites
3' ss Seq
CTGCCCCTTTGATCTTCCAGGCC
3' ss Score
11.02
5' ss Seq
ACAGTACGT
5' ss Score
7.54
Exon sequences
Seq C1 exon
AAATGCTGCCCATGGGTTCTCCCTCATCCAGGTGGACAACACGAAGGTCACCATGAAGGAGATCTTACTCAAGGCACTGAAACGAAGAAAAGGGTCCCAGAAAATTTCAG
Seq A exon
GCCCTCAGTACCGCCTGGAGAAGCAGAGCGAGCCTAACATCGGTGTGGACCTGGAGAGCACGCTGGAGAGCCAGAATGCCTGGGAGTTCTGCCTGGTTCGAGAGAACA
Seq C2 exon
GTTCAAGGGCAGACGGAGTTTTCGAGGAGGATTCACAAATTGACATTGCTACAGTGCAGGATATGCTTAGCAGCCACCATTATAAGTCATTCAAAGTCAGCATGATCCACAGACTGCGGTTCACAACTGACGTGCAGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000017583_MULTIEX2-1/3=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.026 A=0.324 C2=0.000
Domain overlap (PFAM):
C1:
PF054227=SIN1=FE(8.0=100)
A:
PF054227=SIN1=FE(7.8=100)
C2:
PF054227=SIN1=FE(10.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAATGCTGCCCATGGGTTCTC
R:
CGTCAGTTGTGAACCGCAGTC
Band lengths:
242-350
Functional annotations
There are 1 annotated functions for this event
PMID: 16919458
[No functional differences found]. Immunoprecipitation demonstrates that mSin1.1 (all included), mSin1.2 (skipping of HsaEX0037808), and mSin1.5 (AI and APA) integrate into mTORC2 complexes. Insulin treatment increases the kinase activity of mSin1.1 and mSin1.2 but not mSin1.5 complexes.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]