BtaEX0025445 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000019146 | OSBP2
Description
oxysterol binding protein 2 [Source:HGNC Symbol;Acc:HGNC:8504]
Coordinates
chr17:71882274-71883659:+
Coord C1 exon
chr17:71882274-71882322
Coord A exon
chr17:71882499-71882645
Coord C2 exon
chr17:71883414-71883659
Length
147 bp
Sequences
Splice sites
3' ss Seq
GAACTCCGCGTCCCTGGCAGGTG
3' ss Score
8.13
5' ss Seq
CCGGTAGGG
5' ss Score
7.63
Exon sequences
Seq C1 exon
AAAAGCTGAGGCAGGGACTGCAGGCTCCGTGGAATGGACCGCAGACAAT
Seq A exon
GTGCTGGATGGCGCCTCGCCTGTGCCCCAGGGGCCCCCCAAAGTCAAGAGGCGTGTCCGCATCCCTGACAAGCCCAACTACAGCCTTAATCTCTGGAGCATCATGAAGAACTGCATCGGCCGGGAGCTCTCCAAAATCCCCATGCCG
Seq C2 exon
GTGAACTTCAACGAGCCCCTGTCCATGCTCCAGCGGCTGACGGAGGACCTGGAGTACCACCGCCTGCTGGACGCGGCGGCGCGCTGCGGCAGCACAGTGGAGCAGATGTGCCTGGTGGCCGCCTTCTCCGTGTCTTCCTACTCCACCACCGTGCACCGCATCGCCAAGCCCTTCAACCCCATGCTGGGCGAGACCTTCGAGCTGGACCGTCTCGATGACATGGGCCTGCGCTCCCTCTGCGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000019146-'22-12,'22-10,23-12
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.882 A=0.429 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0123713=Oxysterol_BP=PU(5.0=38.8)
C2:
PF0123713=Oxysterol_BP=FE(21.3=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGGAATGGACCGCAGACAAT
R:
GCAGGCCCATGTCATCGAG
Band lengths:
250-397
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development