DreEX0006477 @ danRer10
Exon Skipping
Gene
ENSDARG00000022772 | OSBP2 (1 of 2)
Description
oxysterol binding protein 2 [Source:HGNC Symbol;Acc:HGNC:8504]
Coordinates
chr5:24077370-24082495:-
Coord C1 exon
chr5:24082429-24082495
Coord A exon
chr5:24080735-24080887
Coord C2 exon
chr5:24077370-24077615
Length
153 bp
Sequences
Splice sites
3' ss Seq
TCTCTCTCTGTCTTTTTTAGTGT
3' ss Score
12.76
5' ss Seq
CCTGTAAGT
5' ss Score
7.52
Exon sequences
Seq C1 exon
GCGTTCTCAGAGTAATCTCAGCGGTGCTAGTGGAGGACAGCCCAGTGACTGGAACCAGGATGACAAT
Seq A exon
TGTTCTGTGAGCAGTAATGACTTTTCAGGGAAAGAGCTGCAGCCTCGCAGGAAGAGGCGATCCCGGATCTCAGAGAAACCCAACTATTCCCTCAATCTGTGGAGCATCATGAAGAACTGCATTGGGAAAGAGCTCTCCAAGATCCCAATGCCT
Seq C2 exon
GTGAACTTCAACGAGCCTCTGTCCATGCTGCAGCGTTTGACGGAGGATCTGGAGTATCATGAGCTGCTGGATAAAGCGGCCCGCTGTGAAAACTCTTTGGAGCAGATGTGTCTGGTCGCTGCCTTCTCTGTGTCCTCATACTCCACCACAGTGCACCGGACGGCCAAGCCCTTCAACCCTCTCCTAGGAGAGACCTATGAACTTGACCGACTGGAAGAATTCGGCTACCGTTCCCTCTGTGAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000022772-'16-14,'16-13,17-14
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.451 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0123713=Oxysterol_BP=PU(5.0=37.3)
C2:
PF0123713=Oxysterol_BP=FE(21.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATCTCAGCGGTGCTAGTGGA
R:
CCTAGGAGAGGGTTGAAGGGC
Band lengths:
242-395
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]