RnoEX6043064 @ rn6
Exon Skipping
Gene
ENSRNOG00000019645 | Osbp2
Description
oxysterol binding protein 2 [Source:RGD Symbol;Acc:1310505]
Coordinates
chr14:83953468-83955150:-
Coord C1 exon
chr14:83955096-83955150
Coord A exon
chr14:83954679-83954825
Coord C2 exon
chr14:83953468-83953713
Length
147 bp
Sequences
Splice sites
3' ss Seq
CATGCCTGTTCTCCCGGCAGGTA
3' ss Score
13.13
5' ss Seq
CCGGTAGGC
5' ss Score
7.85
Exon sequences
Seq C1 exon
AAAGCCTGAGGGTGGACCTGGGACGACCTCTGTGGACTGGACCTCAGCAGATAAT
Seq A exon
GTATTAGATGGTGCCTCATTCATGCCCAAGGATTCATGCAAAGTGAAGAGGCGAGTCCGGATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATCATGAAGAATTGCATTGGCCGAGAGCTCTCCCGGATCCCCATGCCG
Seq C2 exon
GTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGACTTACAGAGGACCTCGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTCTGTGGAGCAGATGTGCCTGGTGGCTGCCTTCTCGGTGTCCTCCTACTCCACCACCGTGCACCGCATCGCCAAGCCCTTCAACCCGATGCTGGGGGAGACCTTCGAGTTGGATCGCCTGGAGGACATGGGCCTGCGTTCTCTCTGCGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000019645-'39-28,'39-23,42-28=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.789 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0123713=Oxysterol_BP=PU(5.0=38.8)
C2:
PF0123713=Oxysterol_BP=FE(21.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACGACCTCTGTGGACTGGAC
R:
GCGATCCAACTCGAAGGTCTC
Band lengths:
244-391
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]