MmuEX0033183 @ mm9
Exon Skipping
Gene
ENSMUSG00000020435 | Osbp2
Description
oxysterol binding protein 2 [Source:MGI Symbol;Acc:MGI:1921559]
Coordinates
chr11:3614474-3615554:-
Coord C1 exon
chr11:3615500-3615554
Coord A exon
chr11:3615091-3615237
Coord C2 exon
chr11:3614474-3614719
Length
147 bp
Sequences
Splice sites
3' ss Seq
CGTGCCTGTTCCTCTGGCAGGTA
3' ss Score
11.41
5' ss Seq
CCGGTAGGC
5' ss Score
7.85
Exon sequences
Seq C1 exon
AAAGCCTGAGAGTGGACCTGGGACCACCACTGTGGACTGGACCTCAGCAGATAAT
Seq A exon
GTATTAGATGGTGCCTCATTCATGCCCAAGAATTCATGCAAAGTTAAGAGGCGAGTCCGCATCCCTGACAAACCCAACTATAGCCTTAACCTCTGGAGCATCATGAAGAATTGTATTGGCCGAGAGCTTTCCCGGATCCCCATGCCG
Seq C2 exon
GTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGACTTACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCGGTGAACTGCACCAGCTCGGTGGAGCAGATGTGCCTGGTAGCCGCCTTTTCTGTGTCCTCCTACTCCACCACGGTGCACCGCATCGCCAAGCCCTTCAACCCTATGCTCGGGGAGACCTTCGAGCTGGACCGTATGGAGGACATGGGCCTGCGTTCCCTCTGTGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020435_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.789 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0123713=Oxysterol_BP=PU(5.0=38.8)
C2:
PF0123713=Oxysterol_BP=FE(21.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCACTGTGGACTGGACCTCA
R:
CCATGTCCTCCATACGGTCCA
Band lengths:
252-399
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: