GgaEX7009355 @ galGal4
Exon Skipping
Gene
ENSGALG00000007758 | OSBP2
Description
oxysterol binding protein 2 [Source:HGNC Symbol;Acc:HGNC:8504]
Coordinates
chr15:10705949-10708847:-
Coord C1 exon
chr15:10708781-10708847
Coord A exon
chr15:10708494-10708658
Coord C2 exon
chr15:10705949-10706194
Length
165 bp
Sequences
Splice sites
3' ss Seq
TCCTCCTCTTGCTTTTGCAGGTA
3' ss Score
12.83
5' ss Seq
CCTGTAAGT
5' ss Score
7.52
Exon sequences
Seq C1 exon
GCGTTCGGGCAGTAACCTGAGTGGGGCCAGCGAGGGCCACCCTGAGGACTGGAACCTGGAGGACAGC
Seq A exon
GTATTTGAAAGCTCGAATCAAAGCAGCTATAATGAGTCCTGCAAAGATCTCCTGCCAAAGAAAAGGAGACGGAGCCGCATCCCTGACAAACCCAACTACAGCCTTAACCTCTGGAGCATCATGAAGAACTGCATTGGCAAAGAGCTCTCCAAGATCCCCATGCCT
Seq C2 exon
GTGAACTTCAATGAGCCGCTCTCCATGCTGCAGCGGCTGACGGAGGACCTGGAGTACCACGAGCTGCTGGACAAAGCAGTCAAGTGTGAGAGCTCCACAGAGCAGATGTGTTTCGTGGCAGCCTTTTCGGTGTCTTCCTACTCGACGACGGTGCACCGTACAGCGAAGCCGTTCAACCCGCTGCTGGGGGAAACCTATGAGCTCGACAGGCTGGAGGAGCTGGGCTTCCGTTCCCTGTGTGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000007758-'25-12,'25-10,26-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.200 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0123713=Oxysterol_BP=PU(5.0=34.5)
C2:
PF0123713=Oxysterol_BP=FE(21.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAGGACTGGAACCTGGAGG
R:
CTCCAGCCTGTCGAGCTCATA
Band lengths:
242-407
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]