BtaEX0029470 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000012784 | RACGAP1
Description
Rac GTPase activating protein 1 [Source:HGNC Symbol;Acc:HGNC:9804]
Coordinates
chr5:30048673-30051166:+
Coord C1 exon
chr5:30048673-30048742
Coord A exon
chr5:30050372-30050425
Coord C2 exon
chr5:30051086-30051166
Length
54 bp
Sequences
Splice sites
3' ss Seq
TACAGTACAATTGTCAACAGGAT
3' ss Score
3.46
5' ss Seq
AGGGTATGT
5' ss Score
8.34
Exon sequences
Seq C1 exon
ACTGTCAACCATTGATGAATCTGGTTCCATTTTATCCGATATCAGCTTTGACAAGACCGATGAATCCCTG
Seq A exon
GATTGGGATTCTTCTTTGGTAAAGACTTTTAAACTGAAGAAGAGAGAAAAGAGG
Seq C2 exon
CGCTCAAATAGCCGACAGTTCGTGGATGGTCCCCCTGGACCTGTAAAGAAAACGCGTTCCATTGGCTCCACCGCAGACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012784-'5-11,'5-9,6-11
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.167 A=0.167 C2=1.000
Domain overlap (PFAM):
C1:
PF078886=CALCOCO1=FE(13.7=100),PF140731=Cep57_CLD=PD(9.4=50.0)
A:
PF078886=CALCOCO1=PD(7.7=72.2)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAACCATTGATGAATCTGGTTCCA
R:
GAGCCAATGGAACGCGTTTTC
Band lengths:
134-188
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
PMID: 19468302
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, fluorescence technology, mutation analysis, mutation disrupting interaction, protein kinase assay, western blot. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development