DreEX0060450 @ danRer10
Exon Skipping
Gene
ENSDARG00000015460 | racgap1
Description
Rac GTPase activating protein 1 [Source:ZFIN;Acc:ZDB-GENE-030131-1917]
Coordinates
chr23:33844702-33845173:-
Coord C1 exon
chr23:33845104-33845173
Coord A exon
chr23:33844864-33844917
Coord C2 exon
chr23:33844702-33844779
Length
54 bp
Sequences
Splice sites
3' ss Seq
TTTTTTCAATAACTTTGAAGGAC
3' ss Score
5.85
5' ss Seq
AGAGTAAGC
5' ss Score
6.31
Exon sequences
Seq C1 exon
ACTGGCTACCATTGATGAATCAGCCTCTATCTTGTCAGACATCAGCTATGACAAAACGGATGATTCTTTG
Seq A exon
GACTGGGACTCTTCTGCAATCAGAACCGTTCGTCTTAAGAAACGGCAAAAGAGA
Seq C2 exon
CGCTCTTCCCGAAACCACACCGAAGGACCTCCAGCTGCTGCTAAAAGGTCTCGCTCCACCGGCCGGACATCAGAGAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015460-'5-5,'5-4,6-5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.042 A=0.361 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCTACCATTGATGAATCAGCC
R:
TTTCTCTGATGTCCGGCCGG
Band lengths:
146-200
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
PMID: 19468302
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, fluorescence technology, mutation analysis, mutation disrupting interaction, protein kinase assay, western blot. ELM ID: ELMI003371; ELM sequence: WDSSLVK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]