DreEX0060454 @ danRer10
Exon Skipping
Gene
ENSDARG00000015460 | racgap1
Description
Rac GTPase activating protein 1 [Source:ZFIN;Acc:ZDB-GENE-030131-1917]
Coordinates
chr23:33844864-33847042:-
Coord C1 exon
chr23:33846840-33847042
Coord A exon
chr23:33845104-33845173
Coord C2 exon
chr23:33844864-33844917
Length
70 bp
Sequences
Splice sites
3' ss Seq
ATTCACAACATCTCTTCTAGACT
3' ss Score
4.69
5' ss Seq
TTGGTATGC
5' ss Score
5.91
Exon sequences
Seq C1 exon
AGTTCATTCAGCTGGCCATAAACTTTGAGGACAACCGTCGTAGATGGCTGAGACTCGAACAGGAGCTGAATGCATGCAAAGAAGTGCTCACTAAAGCTGAGACGGAGCGTGGAGCTCTGGAGGTCAAGCTCAAACATGCTCGTAACCAAGTAGATGTGGAGATCCGTAGAAGACAGAAAGCCGAGTCTGACTGCGCAAAGCTG
Seq A exon
ACTGGCTACCATTGATGAATCAGCCTCTATCTTGTCAGACATCAGCTATGACAAAACGGATGATTCTTTG
Seq C2 exon
GACTGGGACTCTTCTGCAATCAGAACCGTTCGTCTTAAGAAACGGCAAAAGAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015460_MULTIEX1-2/2=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.042 C2=0.361
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGGAGCTGAATGCATGCAA
R:
AGACGAACGGTTCTGATTGCA
Band lengths:
180-250
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003372; ELM sequence: SDISFDK; Overlap: FULL
PMID: 27453045
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, isothermal titration calorimetry. ELM ID: ELMI003173; ELM sequence: LSTIDE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]