Special

MmuEX0038469 @ mm9

Exon Skipping

Gene
ENSMUSG00000023015 | Racgap1
Description
Rac GTPase-activating protein 1 [Source:MGI Symbol;Acc:MGI:1349423]
Coordinates
chr15:99464673-99468205:-
Coord C1 exon
chr15:99468069-99468205
Coord A exon
chr15:99466563-99466632
Coord C2 exon
chr15:99464673-99464726
Length
70 bp
Sequences
Splice sites
3' ss Seq
TGTTACTGTTTTTAATCCAGACT
3' ss Score
6.47
5' ss Seq
CTGGTAATA
5' ss Score
4.88
Exon sequences
Seq C1 exon
GAACAACAGATTCAGCTGATTCGAGACATACTCATGTGTGACACATCTGGCAGTATTCAGCTGAGCGAGGAACAAAAATCAGCTCTCGCTTTCCTCAACCGAGGCCAAGCATCCAGTGGCCACGCCGGCAACAATAG
Seq A exon
ACTGTCAACGATTGATGAATCTGGTTCCATTTTATCAGATATCAGCTTTGACAAGACTGATGAATCACTG
Seq C2 exon
GACTGGGATTCTTCTTTGGTGAAGAATTTCAAAATGAAGAAACGAGAGAAGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000023015_MULTIEX1-3/7=2-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.203 A=0.150 C2=0.326
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF059116=DUF869=FE(33.3=100),PF054786=Prominin=FE(31.2=100)

							
A:
PF059116=DUF869=PD(2.2=12.5),PF054786=Prominin=PD(7.6=45.8)

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000023756





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000126417, ENSMUSP00000130876, ENSMUSP00000131182, ENSMUSP00000132732
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr12:50004235-50004304 
HIGH PSI
HsaEX0051857
(RACGAP1)
Human
(hg19)
chr12:50398018-50398087 
HIGH PSI
HsaEX0051857
(RACGAP1)
Rat
(rn6)
chr7:141292830-141292899 
HIGH PSI
RnoEX0073487
(Racgap1)
Cow
(bosTau6)
chr5:30048673-30048742 
Chicken
(galGal4)
chrLGE22C19W28_E50C23:338049-338118 
HIGH PSI
GgaEX1047864
(RACGAP1)
Chicken
(galGal3)
chrE22C19W28_E50C23:318014-318083 
HIGH PSI
GgaEX6046990
(RACGAP1)
Zebrafish
(danRer10)
chr23:33845104-33845173 
HIGH PSI
DreEX0060454
(racgap1)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CGAGACATACTCATGTGTGACACA

				
R: 
CCTCTTCTCTCGTTTCTTCATTTTGA

				
Band lengths: 
170-240

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 2 annotated functions for this event 


PMID: 19468300
HsaEX0051857
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003372; ELM sequence: SDISFDK; Overlap: FULL


PMID: 27453045
HsaEX0051857
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, isothermal titration calorimetry. ELM ID: ELMI003173; ELM sequence: LSTIDE; Overlap: FULL


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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