RnoEX0073487 @ rn6
Exon Skipping
Gene
ENSRNOG00000049033 | Racgap1
Description
Rac GTPase-activating protein 1 [Source:RGD Symbol;Acc:1305912]
Coordinates
chr7:141290976-141294270:-
Coord C1 exon
chr7:141294134-141294270
Coord A exon
chr7:141292830-141292899
Coord C2 exon
chr7:141290976-141291026
Length
70 bp
Sequences
Splice sites
3' ss Seq
TGTTACTGTTTTTAATCCAGACT
3' ss Score
6.47
5' ss Seq
CTGGTAATA
5' ss Score
4.88
Exon sequences
Seq C1 exon
GAACAACAGATTCAGCTGATTCGAGATATACTCATGTGTGACACAACTGGCAGCATCCAGCTAAGCGAGGAACAAAAATCAGCTCTGGCTTTTCTCAACCGAGGCCAAGCATCCAGTGGCAACGCTGGGAACAATAG
Seq A exon
ACTGTCAACGATTGATGAATCTGGCTCCATTTTATCAGATATCAGCTTTGACAAGACTGATGAATCACTG
Seq C2 exon
GACTGGGATTCTTCTTTGAAGAATTTCAAAATGAAGAAACGAGAAAAGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000049033-'12-10,'12-7,14-10
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.181 A=0.139 C2=0.471
Domain overlap (PFAM):
C1:
PF059116=DUF869=FE(32.4=100)
A:
PF059116=DUF869=PD(5.0=29.2)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGATTCAGCTGATTCGAGAT
R:
CCTCTTTTCTCGTTTCTTCATTTTGA
Band lengths:
183-253
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003372; ELM sequence: SDISFDK; Overlap: FULL
PMID: 27453045
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, isothermal titration calorimetry. ELM ID: ELMI003173; ELM sequence: LSTIDE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]