GgaEX1047864 @ galGal4
Exon Skipping
Gene
ENSGALG00000006271 | RACGAP1
Description
Rac GTPase activating protein 1 [Source:HGNC Symbol;Acc:HGNC:9804]
Coordinates
chrLGE22C19W28_E50C23:337713-338372:+
Coord C1 exon
chrLGE22C19W28_E50C23:337713-337849
Coord A exon
chrLGE22C19W28_E50C23:338049-338118
Coord C2 exon
chrLGE22C19W28_E50C23:338319-338372
Length
70 bp
Sequences
Splice sites
3' ss Seq
TTCTAATTCTTGGTTTTTAGACT
3' ss Score
6.99
5' ss Seq
CTGGTAATG
5' ss Score
5.67
Exon sequences
Seq C1 exon
GAGAGGCAAATACAGCTGATTCGAGAGCTGCTCATGTGTGATGCATCTGGGAGCATTCAGCTAAGTGAAGAACAGAAGTCTGCCCTTGCCTTCCTTAACAGACCACAGGTTTCTGTAGGAGGTTCAGGCAATAAGAG
Seq A exon
ACTGTCTACAATAGATGAATCTGGCTCAATTCTGTCAGACATCAGCTTTGACAAGACTGATGATTCACTG
Seq C2 exon
GACTGGGATTCCTCTGCGGTGAAGGCTGTCAGACTGAAGAGGAGAGAGAAGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000006271-'17-6,'17-5,18-6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.109 A=0.042 C2=0.222
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=PD(22.5=67.4)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTGATTCGAGAGCTGCTCA
R:
TCCTCTTCAGTCTGACAGCCT
Band lengths:
167-237
Functional annotations
There are 2 annotated functions for this event
PMID: 19468300
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, mutation analysis, protein kinase assay. ELM ID: ELMI003372; ELM sequence: SDISFDK; Overlap: FULL
PMID: 27453045
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, isothermal titration calorimetry. ELM ID: ELMI003173; ELM sequence: LSTIDE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]